ArticleInfection and immunity2000
Enhanced production of recombinant Mycobacterium tuberculosis antigens in Escherichia coli by replacement of low-usage codons.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- Cyclipostins and cyclophostin analogs inhibit the antigen 85C fromThe Journal of biological chemistry · 2018Article
- An Engineered Rare Codon Device for Optimization of Metabolic Pathways.Scientific reports · 2016Article
- Codon Usage in Signal Sequences Affects Protein Expression and Secretion Using Baculovirus/Insect Cell Expression System.PloS one · 2015Article
- A comparative approach to strategies for cloning, expression, and purification of Mycobacterium tuberculosis mycolyl transferase 85B and evaluation of immune responses in BALB/c mice.Molecular biotechnology · 2014Article
- Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins.Microbial cell factories · 2013Article
- Characterization of MHC-II antigen presentation by B cells and monocytes from older individuals.Clinical immunology (Orlando, Fla.) · 2012Article
- Type I IFN drives a distinctive dendritic cell maturation phenotype that allows continued class II MHC synthesis and antigen processing.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Article
- Pulmonary immunization using antigen 85-B polymeric microparticles to boost tuberculosis immunity.The AAPS journal · 2010Article
- Rational design of multiple TB antigens TB10.4 and TB10.4-Ag85B as subunit vaccine candidates against Mycobacterium tuberculosis.Pharmaceutical research · 2010Article
- A novel bicistronic vector for overexpressing Mycobacterium tuberculosis proteins in Escherichia coli.Protein expression and purification · 2009Article
- Reducing the activity and secretion of microbial antioxidants enhances the immunogenicity of BCG.PloS one · 2009Article
- Poly (lactide-co-glycolide) microspheres in respirable sizes enhance an in vitro T cell response to recombinant Mycobacterium tuberculosis antigen 85B.Pharmaceutical research · 2007Article
- High level expression of recombinant Mycobacterium tuberculosis culture filtrate protein CFP32 in Pichia pastoris.Molecular biotechnology · 2007Article
- Class II MHC antigen presentation defect in neonatal monocytes is not correlated with decreased MHC-II expression.Cellular immunology · 2006Article
- Inhibition of translation by consecutive rare leucine codons in E. coli: absence of effect of varying mRNA stability.Gene expression · 2006Article
- Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes.World journal of gastroenterology · 2005Article
- Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli.Protein science : a publication of the Protein Society · 2005Article
- The Mycobacterium tuberculosis 19-kilodalton lipoprotein inhibits gamma interferon-regulated HLA-DR and Fc gamma R1 on human macrophages through Toll-like receptor 2.Infection and immunity · 2003Article
- mRNA differential display in a microbial enrichment culture: simultaneous identification of three cyclohexanone monooxygenases from three species.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
A major obstacle to development of subunit vaccines and diagnostic reagents for tuberculosis is the inability to produce large quantities of these proteins. To test the hypothesis that poor expression of some mycobacterial genes in Escherichia coli is due, in part, to the presence of low-usage E. coli codons, we used site-directed mutagenesis to convert low-usage codons to high-usage codons for the same amino acid in the Mycobacterium tuberculosis genes for antigens 85A and 85B and superoxide dismutase. Replacement of five codons in the wild-type gene for antigen 85B increased recombinant protein production in E. coli 54-fold. The recombinant antigen elicited proliferation and gamma interferon production by lymphocytes from healthy tuberculin reactors and was recognized by monoclonal antibodies to native antigen 85, indicating that the recombinant antigen contained T-cell and B-cell epitopes. Northern blotting demonstrated only a 1.7- to 2.5-fold increase in antigen 85B mRNA, suggesting that the enhanced protein production was due primarily to enhanced efficiency of translation. Codon replacement in the genes encoding antigen 85A and superoxide dismutase yielded four- to sixfold increases in recombinant protein production, suggesting that this strategy may be generally applicable to overexpression of mycobacterial genes in E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.