ArticleInfection and immunity2000
Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers.
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163 citing papers in PubMed.
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- Pathogenomes of Shiga Toxin Positive and NegativeMicroorganisms · 2024Article
- Multidrug resistance in pathogenicFrontiers in veterinary science · 2024Article
- Microbiological safety and antimicrobial resistance profiles of ready-to-eat seafood in Bayelsa, Nigeria.Sustainable microbiology · 2024Article
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- EnteroaggregativeFrontiers in microbiology · 2023Article
- Prevalence and Characterization of Shiga Toxin ProducingMicroorganisms · 2022Article
- First study on virulence genes, antimicrobial resistance, and integrons inVeterinary world · 2022Article
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- Characterization of the Emerging EnteropathogenInternational journal of microbiology · 2022Article
- Genomic Diversity, Virulence Gene, and Prophage Arrays of Bovine and Human Shiga Toxigenic and EnteropathogenicFrontiers in microbiology · 2022Article
- Added Value of Genomic Surveillance of Virulence Factors in Shiga Toxin-ProducingFrontiers in microbiology · 2021Article
- Molecular characteristics ofEmerging microbes & infections · 2020Article
- Genetic diversity of the intimin gene (eae) in non-O157 Shiga toxin-producing Escherichia coli strains in China.Scientific reports · 2020Article
- Captive Green Iguana Carries DiarrheagenicFrontiers in veterinary science · 2020Article
- Prevalence and virulence gene profiles of Escherichia coli O157 from cattle slaughtered in Buea, Cameroon.PloS one · 2020Article
103 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) produce the characteristic "attaching and effacing" (A/E) lesion of the brush border. Intimin, an outer membrane protein encoded by eae, is responsible for the tight association of both pathogens with the host cell. Several eae have been cloned from different EPEC and EHEC strains isolated from humans and animals. These sequences are conserved in the N-terminal region but highly variable in the last C-terminal 280 amino acids (aa), where the cell binding activity is localized. Based on these considerations, we developed a panel of specific primers to investigate the eae heterogeneity of the variable 3' region by using PCR amplification. We then investigated the distribution of the known intimin types in a large collection of EPEC and EHEC strains isolated from humans and different animal species. The existence of a yet-unknown family of intimin was suspected because several EHEC strains, isolated from human and cattle, did not react with any of the specific primer pairs, although these strains were eae positive when primers amplifying the conserved 5' end were used. We then cloned and sequenced the eae present in one of these strains (EHEC of serotype O103:H2) and subsequently designed a PCR primer that recognizes in a specific manner the variable 3' region of this new intimin type. This intimin, referred to as "epsilon," was present in human and bovine EHEC strains of serogroups O8, O11, O45, O103, O121, and O165. Intimin epsilon is the largest intimin cloned to date (948 aa) and shares the greatest overall sequence identity with intimin beta, although analysis of the last C-terminal 280 aa suggests a greater similarity with intimins alpha and gamma.
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