ArticleGene therapy1999
Mechanisms of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides.
Article in Gene therapy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 163 citations in OpenAlex.
- Lysosomal Storage Disease-Associated Neuropathy: Targeting Stable Nucleic Acid Lipid Particle (SNALP)-Formulated siRNAs to the Brain as a Therapeutic Approach.International journal of molecular sciences · 2020Review
- Application of advances in endocytosis and membrane trafficking to drug delivery.Advanced drug delivery reviews · 2020Review
- Lipid-peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery.Journal of nanobiotechnology · 2019Article
- Non-viral Delivery of Nucleic Acids: Insight Into Mechanisms of Overcoming Intracellular Barriers.Frontiers in pharmacology · 2018Review
- Gene Delivery Approaches for Mesenchymal Stem Cell Therapy: Strategies to Increase Efficiency and Specificity.Stem cell reviews and reports · 2017Review
- Journey to the Center of the Cell: Current Nanocarrier Design Strategies Targeting Biopharmaceuticals to the Cytoplasm and Nucleus.Current pharmaceutical design · 2016Review
- Combined treatment with a pH-sensitive fusogenic peptide and cationic lipids achieves enhanced cytosolic delivery of exosomes.Scientific reports · 2015Article
- Efficient protection and transfection of small interfering RNA by cationic shell-crosslinked knedel-like nanoparticles.Nucleic acid therapeutics · 2013Article
- Novel nanosomes for gene delivery to Plasmodium falciparum-infected red blood cells.Scientific reports · 2013Article
- Cationic liposome/DNA complexes: from structure to interactions with cellular membranes.European biophysics journal : EBJ · 2012Review
- Coupled folding and specific binding: fishing for amphiphilicity.International journal of molecular sciences · 2011Review
- Incorporation of 2,3-diaminopropionic acid into linear cationic amphipathic peptides produces pH-sensitive vectors.Chembiochem : a European journal of chemical biology · 2010Article
- Cell-Penetrating Peptides-Mechanisms of Cellular Uptake and Generation of Delivery Systems.Pharmaceuticals (Basel, Switzerland) · 2010Review
- Transferrin lipoplex-mediated suicide gene therapy of oral squamous cell carcinoma in an immunocompetent murine model and mechanisms involved in the antitumoral response.Cancer gene therapy · 2009Article
- Targeted cationic poly(D,L-lactic-co-glycolic acid) nanoparticles for gene delivery to cultured cells.Cellular & molecular biology letters · 2009Article
- Transferrin-associated lipoplexes as gene delivery systems: relevance of mode of preparation and biophysical properties.The Journal of membrane biology · 2008Article
- Gene delivery by cationic lipid vectors: overcoming cellular barriers.European biophysics journal : EBJ · 2007Review
- Intracellular trafficking of nonviral vectors.Gene therapy · 2005Review
- Tf-lipoplex-mediated NGF gene transfer to the CNS: neuronal protection and recovery in an excitotoxic model of brain injury.Gene therapy · 2005Article
- Polymers for DNA delivery.Molecules (Basel, Switzerland) · 2005Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Association of a targeting ligand such as transferrin, or an endosome disrupting peptide such as GALA, with cationic liposome-DNA complexes ('lipoplexes') results in a significant enhancement of transfection of several cell types (Simões S et al, Gene Therapy 1998; 5: 955-964). Although these strategies can overcome some of the barriers to gene delivery by lipoplexes, the mechanisms by which they actually enhance tranfection is not known. In studies designed to establish the targeting specificity of transferrin, we found that apo-transferrin enhances transfection to the same extent as transferrin, indicating that internalization of the lipoplexes is mostly independent of transferrin receptors. These observations were reinforced by results obtained from competitive inhibition studies either by preincubating the cells with an excess of free ligand or with various 'receptor-blocking' lipoplexes. Transfection of cells in the presence of drugs that interfere with the endocytotic pathway provided additional insights into the mechanisms of gene delivery by transferrin- or GALA-lipoplexes. Our results indicate that transferrin-lipoplexes deliver transgenes by endocytosis primarily via a non-receptor-mediated mechanism, and that acidification of the endosomes is partially involved in this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.