ArticleThe Journal of cell biology1999
Keratocytes pull with similar forces on their dorsal and ventral surfaces.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 98 citations in OpenAlex.
- Diversity in emergent cell locomotion from the coupling cytosolic and cortical Marangoni flows with reaction-diffusion dynamics.PLoS computational biology · 2026Article
- Versatile and High-throughput Force Measurement Platform for Dorsal Cell Mechanics.Scientific reports · 2019Article
- The desmoplakin-intermediate filament linkage regulates cell mechanics.Molecular biology of the cell · 2017Article
- Interplay of RhoA and mechanical forces in collective cell migration driven by leader cells.Nature cell biology · 2014Article
- Regulation of RhoA activity by adhesion molecules and mechanotransduction.Current molecular medicine · 2014Review
- Cell migration.Comprehensive Physiology · 2012Review
- Membrane tension, myosin force, and actin turnover maintain actin treadmill in the nerve growth cone.Biophysical journal · 2012Article
- Traction forces of neutrophils migrating on compliant substrates.Biophysical journal · 2011Article
- Positively and negatively modulating cell adhesion to type I collagen via peptide grafting.Tissue engineering. Part A · 2011Article
- A POROELASTIC MODEL FOR CELL CRAWLING INCLUDING MECHANICAL COUPLING BETWEEN CYTOSKELETAL CONTRACTION AND ACTIN POLYMERIZATION.Journal of mechanics of materials and structures · 2011Article
- Leading tip drives soma translocation via forward F-actin flow during neuronal migration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Article
- A microfluidic imaging chamber for the direct observation of chemotactic transmigration.Biomedical microdevices · 2010Article
- Probing cellular traction forces by micropillar arrays: contribution of substrate warping to pillar deflection.Nano letters · 2010Article
- Cytoskeletal coherence requires myosin-IIA contractility.Journal of cell science · 2010Article
- Force transmission in migrating cells.The Journal of cell biology · 2010Article
- Actin-myosin viscoelastic flow in the keratocyte lamellipod.Biophysical journal · 2009Article
- Locomotion guidance by extracellular matrix is adaptive and can be restored by a transient change in Ca2+ level.PloS one · 2009Article
- Intracellular fluid flow in rapidly moving cells.Nature cell biology · 2009Article
- Weak force stalls protrusion at the leading edge of the lamellipodium.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
As cells move forward, they pull rearward against extracellular matrices (ECMs), exerting traction forces. However, no rearward forces have been seen in the fish keratocyte. To address this discrepancy, we have measured the propulsive forces generated by the keratocyte lamella on both the ventral and the dorsal surfaces. On the ventral surface, a micromachined device revealed that traction forces were small and rearward directed under the lamella, changed direction in front of the nucleus, and became larger under the cell body. On the dorsal surface of the lamella, an optical gradient trap measured rearward forces generated against fibronectin-coated beads. The retrograde force exerted by the cell on the bead increased in the thickened region of the lamella where myosin condensation has been observed (Svitkina, T.M., A.B. Verkhovsky, K.M. McQuade, and G. G. Borisy. 1997. J. Cell Biol. 139:397-415). Similar forces were generated on both the ventral (0.2 nN/microm(2)) and the dorsal (0.4 nN/microm(2)) surfaces of the lamella, suggesting that dorsal matrix contacts are as effectively linked to the force-generating cytoskeleton as ventral contacts. The correlation between the level of traction force and the density of myosin suggests a model for keratocyte movement in which myosin condensation in the perinuclear region generates rearward forces in the lamella and forward forces in the cell rear.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.