ArticleThe Journal of cell biology1999
Dynein intermediate chain mediated dynein-dynactin interaction is required for interphase microtubule organization and centrosome replication and separation in Dictyostelium.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 94 citations in OpenAlex.
- Dietary variations drive divergent phenotypic, transcriptomic, and metatranscriptomic profiles in Biomphalaria glabrata, a schistosomiasis vector snail.Parasites & vectors · 2026Article
- Genome-scale requirements for dynein-based transport revealed by a high-content arrayed CRISPR screen.The Journal of cell biology · 2024Article
- Structure and tethering mechanism of dynein-2 intermediate chains in intraflagellar transport.The EMBO journal · 2024Article
- Genome-scale requirements for dynein-based trafficking revealed by a high-content arrayed CRISPR screen.bioRxiv : the preprint server for biology · 2023Article
- Katnip is needed to maintain microtubule function and lysosomal delivery to autophagosomes and phagosomes.Molecular biology of the cell · 2023Article
- Article
- On and off controls within dynein-dynactin on native cargoes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Review
- Force balances between interphase centrosomes as revealed by laser ablation.Molecular biology of the cell · 2019Article
- Review
- Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila.Molecular biology and evolution · 2017Article
- Organization of microtubule assemblies in Dictyostelium syncytia depends on the microtubule crosslinker, Ase1.Cellular and molecular life sciences : CMLS · 2016Article
- Emerging roles of sumoylation in the regulation of actin, microtubules, intermediate filaments, and septins.Cytoskeleton (Hoboken, N.J.) · 2015Review
- Analysis of dynein intermediate chains, light intermediate chains and light chains in a cohort of hereditary peripheral neuropathies.Neurogenetics · 2014Article
- Trim58 degrades Dynein and regulates terminal erythropoiesis.Developmental cell · 2014Article
- Exposure to carbon nanotubes leads to changes in the cellular biomechanics.Advanced healthcare materials · 2013Article
- A kinesin-mediated mechanism that couples centrosomes to nuclei.Cellular and molecular life sciences : CMLS · 2013Article
- CP55, a novel key component of centrosomal organization in Dictyostelium.Cellular and molecular life sciences : CMLS · 2012Article
- Single-walled carbon nanotube-induced mitotic disruption.Mutation research · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cytoplasmic dynein intermediate chain (IC) mediates dynein-dynactin interaction in vitro (Karki, S., and E.L. Holzbaur. 1995. J. Biol. Chem. 270:28806-28811; Vaughan, K.T., and R.B. Vallee. 1995. J. Cell Biol. 131:1507-1516). To investigate the physiological role of IC and dynein-dynactin interaction, we expressed IC truncations in wild-type Dictyostelium cells. ICDeltaC associated with dynactin but not with dynein heavy chain, whereas ICDeltaN truncations bound to dynein but bound dynactin poorly. Both mutations resulted in abnormal localization to the Golgi complex, confirming dynein function was disrupted. Striking disorganization of interphase microtubule (MT) networks was observed when mutant expression was induced. In a majority of cells, the MT networks collapsed into large bundles. We also observed cells with multiple cytoplasmic asters and MTs lacking an organizing center. These cells accumulated abnormal DNA content, suggesting a defect in mitosis. Striking defects in centrosome morphology were also observed in IC mutants, mostly larger than normal centrosomes. Ultrastructural analysis of centrosomes in IC mutants showed interphase accumulation of large centrosomes typical of prophase as well as unusually paired centrosomes, suggesting defects in centrosome replication and separation. These results suggest that dynactin-mediated cytoplasmic dynein function is required for the proper organization of interphase MT network as well as centrosome replication and separation in Dictyostelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.