ArticleThe Journal of cell biology1999
The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 135 citations in OpenAlex.
- The ascent of AKAPs, from architectural elements to kinase anchors: a perspective.The Biochemical journal · 2025Article
- Biallelic FBXW7 knockout induces AKAP8-mediated DNA damage in neighbouring wildtype cells.Cell death discovery · 2023Article
- Factor-specific generative pattern from large-scale drug-induced gene expression profile.Scientific reports · 2023Article
- DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network.Molecular cell · 2023Article
- High AKAP8L expression predicts poor prognosis in esophageal squamous cell carcinoma.Cancer cell international · 2022Article
- Review
- Subcellular Organization of the cAMP Signaling Pathway.Pharmacological reviews · 2021Review
- The autophagy protein Ambra1 regulates gene expression by supporting novel transcriptional complexes.The Journal of biological chemistry · 2020Article
- Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis.Nature cell biology · 2020Article
- Cx43 and AKAP95 regulate G1/S conversion by competitively binding to cyclin E1/E2 in lung cancer cells.Thoracic cancer · 2020Article
- Disruption of AKAP-PKA Interaction Induces Hypercontractility With Concomitant Increase in Proliferation Markers in Human Airway Smooth Muscle.Frontiers in cell and developmental biology · 2020Article
- Discovering genetic interactions bridging pathways in genome-wide association studies.Nature communications · 2019Article
- FSH/LH-Dependent Upregulation of Ahr in Murine Granulosa Cells Is Controlled by PKA Signaling and Involves Epigenetic Regulation.International journal of molecular sciences · 2019Article
- AKAP95 Organizes a Nuclear Microdomain to Control Local cAMP for Regulating Nuclear PKA.Cell chemical biology · 2019Article
- AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint.Cell cycle (Georgetown, Tex.) · 2017Article
- AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors.Nature communications · 2016Article
- Dynamic changes in protein interaction between AKAP95 and Cx43 during cell cycle progression of A549 cells.Scientific reports · 2016Article
- Regulation of Calcium-Independent Phospholipase A2 Expression by Adrenoceptors and Sterol Regulatory Element Binding Protein-Potential Crosstalk Between Sterol and Glycerophospholipid Mediators.Molecular neurobiology · 2016Article
- Molecular Mechanisms for cAMP-Mediated Immunoregulation in T cells - Role of Anchored Protein Kinase A Signaling Units.Frontiers in immunology · 2016Review
- Scaffolding during the cell cycle by A-kinase anchoring proteins.Pflugers Archiv : European journal of physiology · 2015Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein kinase A (PKA) and the nuclear A-kinase-anchoring protein AKAP95 have previously been shown to localize in separate compartments in interphase but associate at mitosis. We demonstrate here a role for the mitotic AKAP95-PKA complex. In HeLa cells, AKAP95 is associated with the nuclear matrix in interphase and redistributes mostly into a chromatin fraction at mitosis. In a cytosolic extract derived from mitotic cells, AKAP95 recruits the RIIalpha regulatory subunit of PKA onto chromatin. Intranuclear immunoblocking of AKAP95 inhibits chromosome condensation at mitosis and in mitotic extract in a PKA-independent manner. Immunodepletion of AKAP95 from the extract or immunoblocking of AKAP95 at metaphase induces premature chromatin decondensation. Condensation is restored in vitro by a recombinant AKAP95 fragment comprising the 306-carboxy-terminal amino acids of the protein. Maintenance of condensed chromatin requires PKA binding to chromatin-associated AKAP95 and cAMP signaling through PKA. Chromatin-associated AKAP95 interacts with Eg7, the human homologue of Xenopus pEg7, a component of the 13S condensin complex. Moreover, immunoblocking nuclear AKAP95 inhibits the recruitment of Eg7 to chromatin in vitro. We propose that AKAP95 is a multivalent molecule that in addition to anchoring a cAMP/PKA-signaling complex onto chromosomes, plays a role in regulating chromosome structure at mitosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.