ArticleJournal of bacteriology1999
Citrate synthase mutants of Sinorhizobium meliloti are ineffective and have altered cell surface polysaccharides.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- Deletion ofApplied and environmental microbiology · 2025Article
- How Rhizobia Adapt to the Nodule Environment.Journal of bacteriology · 2021Article
- Interaction and Regulation of Carbon, Nitrogen, and Phosphorus Metabolisms in Root Nodules of Legumes.Frontiers in plant science · 2018Review
- Lipogenesis and Redox Balance in Nitrogen-Fixing Pea Bacteroids.Journal of bacteriology · 2016Article
- Pyruvate is synthesized by two pathways in pea bacteroids with different efficiencies for nitrogen fixation.Journal of bacteriology · 2010Article
- Deletion of citrate synthase restores growth of Sinorhizobium meliloti 1021 aconitase mutants.Journal of bacteriology · 2009Article
- Transcriptomic analysis of Rhizobium leguminosarum biovar viciae in symbiosis with host plants Pisum sativum and Vicia cracca.Journal of bacteriology · 2009Article
- Citrate synthase mutants of Agrobacterium are attenuated in virulence and display reduced vir gene induction.Journal of bacteriology · 2005Article
- Citrate synthase mutants of Sinorhizobium fredii USDA257 form ineffective nodules with aberrant ultrastructure.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resulting strain, CSDX1, was a glutamate auxotroph, and enzyme assays confirmed the absence of a requirement for glutamate. CSDX1 did not grow on succinate, malate, aspartate, pyruvate, or glucose. CSDX1 produced an unusual blue fluorescence on medium containing Calcofluor, which is different from the green fluorescence found with 104A14. High concentrations of arabinose (0.4%) or succinate (0. 2%) restored the green fluorescence to CSDX1. High-performance liquid chromatography analyses showed that CSDX1 produced partially succinylated succinoglycan. CSDX1 was able to form nodules on alfalfa, but these nodules were not able to fix nitrogen. The symbiotic defect of a citrate synthase mutant could thus be due to disruption of the infection process or to the lack of energy generated by the tricarboxylic acid cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.