ArticleJournal of bacteriology1999
Differential and independent roles of a sigma(32) homolog (RpoH) and an HrcA repressor in the heat shock response of Agrobacterium tumefaciens.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- The Role ofInternational journal of molecular sciences · 2025Review
- The Role of Heat Shock Protein (Hsp) Chaperones in Environmental Stress Adaptation and Virulence of Plant Pathogenic Bacteria.International journal of molecular sciences · 2025Review
- Temporal evolution of master regulator Crp identifies pyrimidines as catabolite modulator factors.Nature communications · 2021Article
- Arginine-Rich Small Proteins with a Domain of Unknown Function, DUF1127, Play a Role in Phosphate and Carbon Metabolism of Agrobacterium tumefaciens.Journal of bacteriology · 2020Article
- Expression of Two RpoH Sigma Factors in Sinorhizobium meliloti upon Heat Shock.Microbes and environments · 2017Article
- A Sinorhizobium meliloti RpoH-Regulated Gene Is Involved in Iron-Sulfur Protein Metabolism and Effective Plant Symbiosis under Intrinsic Iron Limitation.Journal of bacteriology · 2016Article
- Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA.Plant cell reports · 2016Article
- Improvement ofPlant biotechnology reports · 2013Article
- Article
- Dual RpoH sigma factors and transcriptional plasticity in a symbiotic bacterium.Journal of bacteriology · 2012Article
- Regulated proteolysis in Gram-negative bacteria--how and when?Nature reviews. Microbiology · 2011Review
- Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers.Plant cell reports · 2011Article
- Global gene expression analysis of the heat shock response in the phytopathogen Xylella fastidiosa.Journal of bacteriology · 2006Article
- Comparison of the RpoH-dependent regulon and general stress response in Neisseria gonorrhoeae.Journal of bacteriology · 2006Article
- Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans.Journal of bacteriology · 2005Article
- Replicon-specific regulation of small heat shock genes in Agrobacterium tumefaciens.Journal of bacteriology · 2004Article
- Sinorhizobium meliloti RpoH1 is required for effective nitrogen-fixing symbiosis with alfalfa.Molecular genetics and genomics : MGG · 2004Article
- Heat shock proteome of Agrobacterium tumefaciens: evidence for new control systems.Journal of bacteriology · 2002Article
- Elevated temperature differentially affects virulence, VirB protein accumulation, and T-pilus formation in different Agrobacterium tumefaciens and Agrobacterium vitis strains.Journal of bacteriology · 2001Article
- DnaK chaperone-mediated control of activity of a sigma(32) homolog (RpoH) plays a major role in the heat shock response of Agrobacterium tumefaciens.Journal of bacteriology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heat shock response in alpha proteobacteria is unique in that a combination of two regulators is involved: a positive regulator, RpoH (sigma(32) homolog), found in the alpha, beta, and gamma proteobacteria, and a negative regulator, HrcA, widely distributed in eubacteria but not in the gamma proteobacteria. To assess the differential roles of the two regulators in these bacteria, we cloned the hrcA-grpE operon of Agrobacterium tumefaciens, analyzed its transcription, and constructed deletion mutants lacking RpoH and/or HrcA. The DeltarpoH mutant and DeltarpoH DeltahrcA double mutant were unable to grow above 30 degrees C. Whereas the synthesis of heat shock proteins (e.g., DnaK, GroEL, and ClpB) was transiently induced upon temperature upshift from 25 to 37 degrees C in the wild type, such induction was not observed in the DeltarpoH mutant, except that GroEL synthesis was still partially induced. By contrast, the DeltahrcA mutant grew normally and exhibited essentially normal heat induction except for a higher level of GroEL expression, especially before heat shock. The DeltarpoH DeltahrcA double mutant showed the combined phenotypes of each of the single mutants. The amounts of dnaK and groE transcripts before and after heat shock, as determined by primer extension, were consistent with those of the proteins synthesized. The cellular level of RpoH but not HrcA increased significantly upon heat shock. We conclude that RpoH plays a major and global role in the induction of most heat shock proteins, whereas HrcA plays a restricted role in repressing groE expression under nonstress conditions.
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