ArticleOncogene1999
Ras pathway is required for the activation of MMP-2 secretion and for the invasion of src-transformed 3Y1.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
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- Flaccidoxide-13-Acetate Extracted from the Soft Coral Cladiella kashmani Reduces Human Bladder Cancer Cell Migration and Invasion through Reducing Activation of the FAK/PI3K/AKT/mTOR Signaling Pathway.Molecules (Basel, Switzerland) · 2017Article
- Local inhibition of angiogenesis by halofuginone coated silicone materials.Journal of materials science. Materials in medicine · 2012Article
- Transcriptional silencing of ETS-1 efficiently suppresses angiogenesis of pancreatic cancer.Cancer gene therapy · 2009Article
- Cia5d regulates a new fibroblast-like synoviocyte invasion-associated gene expression signature.Arthritis research & therapy · 2008Article
- Stat3 activation regulates the expression of matrix metalloproteinase-2 and tumor invasion and metastasis.Oncogene · 2004Article
- Restoration of SMAD4 by gene therapy reverses the invasive phenotype in pancreatic adenocarcinoma cells.Oncogene · 2003Article
- Article
- Hyaluronan activates cell motility of v-Src-transformed cells via Ras-mitogen-activated protein kinase and phosphoinositide 3-kinase-Akt in a tumor-specific manner.Molecular biology of the cell · 2001Article
- A role for FAK in the Concanavalin A-dependent secretion of matrix metalloproteinase-2 and -9.Oncogene · 2000Article
- v-Src suppresses SHPS-1 expression via the Ras-MAP kinase pathway to promote the oncogenic growth of cells.Oncogene · 2000Article
- Fibronectin activates matrix metalloproteinase-9 secretion via the MEK1-MAPK and the PI3K-Akt pathways in ovarian cancer cells.Clinical & experimental metastasis · 2000Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To search for the signaling pathway critical for tumor invasion, we examined the effects of dominant negative ras (S17N ras) expression on the activation of matrix metalloproteinase-2 (MMP-2) in src-transformed 3Y1, SR3Y1, under the control of conditionally inducible promoter. In SR3Y1 clones transfected with S17N ras, augmented secretion and proteolytic activation of MMP-2 were dramatically suppressed by S17N Ras expression, while tyrosine phosphorylation of cellular proteins was not suppressed. We found that invasiveness of SR3Y1 cells assayed by the modified Boyden Chamber method was strongly suppressed by S17N Ras expression. In contrast, cell morphology reverted partially and glucose uptake remained unchanged by S17N Ras expression. In addition, treatment of SR3Y1 with manumycin A, a potent inhibitor of Ras farnesyltransferase, strongly suppressed both augmented secretion and proteolytic activation of MMP-2. Contrary, treatment of SR3Y1 with wortmannin or TPA showed no clear effect on MMP-2 activation. Thus, these results strongly suggest that Ras-signaling, but neither P13 kinase- nor protein kinase C-signalings, plays a critical role in activation of MMP-2 and, subsequently, in the invasiveness of src-transformed cells.
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