ArticleOncogene1999
Association of Pur alpha and E2F-1 suppresses transcriptional activity of E2F-1.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Purine-Rich Element Binding Protein Alpha, a Nuclear Matrix Protein, Has a Role in Prostate Cancer Progression.International journal of molecular sciences · 2024Review
- Effects of In Utero EtOH Exposure on 18S Ribosomal RNA Processing: Contribution to Fetal Alcohol Spectrum Disorder.International journal of molecular sciences · 2023Article
- RETRACTED: Genome-wide identification of RETINOBLASTOMA RELATED 1 binding sites in Arabidopsis reveals novel DNA damage regulators.PLoS genetics · 2018Article
- The purine-rich element-binding protein ChPur-α negatively regulates Hsc70 transcription in Crassostrea hongkongensis.Cell stress & chaperones · 2018Article
- Review
- Article
- Structural basis of multisite single-stranded DNA recognition and ACTA2 repression by purine-rich element binding protein B (Purβ).Biochemistry · 2013Article
- The pur protein family: genetic and structural features in development and disease.Journal of cellular physiology · 2013Review
- The nuclear protein ALY binds to and modulates the activity of transcription factor E2F2.Molecular & cellular proteomics : MCP · 2013Article
- The RB/E2F pathway and regulation of RNA processing.Biochemical and biophysical research communications · 2009Review
- Multiple roles for Puralpha in cellular and viral regulation.Cell cycle (Georgetown, Tex.) · 2009Review
- Protective role of Puralpha to cisplatin.Cancer biology & therapy · 2008Article
- C9orf10 protein, a novel protein component of Puralpha-containing mRNA-protein particles (Puralpha-mRNPs): characterization of developmental and regional expressions in the mouse brain.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Puralpha is essential for postnatal brain development and developmentally coupled cellular proliferation as revealed by genetic inactivation in the mouse.Molecular and cellular biology · 2003Article
- Puralpha: a multifunctional single-stranded DNA- and RNA-binding protein.Nucleic acids research · 2000Review
- Association of HIV-1 Tat with the cellular protein, Puralpha, is mediated by RNA.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Role of Puralpha in the modulation of homologous recombination-directed DNA repair by HIV-1 Tat.Anticancer researchArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-protein interaction can play an important role in the control of several biological events including gene transcription, replication and cell proliferation. E2F-1 is a DNA-binding transcription factor which, upon interaction with its target DNA sequence, induces expression of several S phase specific genes allowing progression of the cell cycle. Evidently, the activity of this protein is modulated by its cellular partner, pRb, which in the hypophosphorylated form, binds to E2F-1 and inactivates its transcriptional ability. In this study, we have demonstrated that expression of a sequence-specific single-stranded DNA binding protein, Pur alpha, in cells decreases the ability of E2F-1 to exert its transcriptional activity upon the responsive promoter derived from DHFR. Results from band shift experiments revealed that while Pur alpha does not recognize the double-stranded DNA fragment containing the E2F-1 binding site, it has the ability to inhibit E2F-1 interaction with its target DNA sequence. Results from GST pull-down assays and the combined immunoprecipitation/Western blot analysis of nuclear extracts revealed a direct association of E2F-1 with Pur alpha in the absence of the DNA molecule containing the E2F-1 binding site. The association of Pur alpha with E2F-1 may increase the stability of E2F-1, as a higher level of E2F-1 was detected in cells coexpressing Pur alpha and E2F-1. The importance of these observations with respect to the role of Pur alpha in the control of cell cycle progression is discussed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.