ArticleOncogene1999
Telomerase inhibition by peptide nucleic acids reverses 'immortality' of transformed human cells.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed.
- Artificial genetic polymers against human pathologies.Biology direct · 2022Review
- Senescence of Tumor Cells in Anticancer Therapy-Beneficial and Detrimental Effects.International journal of molecular sciences · 2022Review
- Possibility of inducing tumor cell senescence during therapy.Oncology letters · 2021Review
- Radiolabeled Oligonucleotides Targeting the RNA Subunit of Telomerase Inhibit Telomerase and Induce DNA Damage in Telomerase-Positive Cancer Cells.Cancer research · 2019Article
- Inhibition of telomerase activity and induction of apoptosis by Rhodospirillum rubrum L-asparaginase in cancer Jurkat cell line and normal human CD4+ T lymphocytes.Cancer medicine · 2017Article
- Inhibitors of telomerase and poly(ADP-ribose) polymerases synergize to limit the lifespan of pancreatic cancer cells.Oncotarget · 2017Article
- Article
- Telomerase inhibitor Imetelstat (GRN163L) limits the lifespan of human pancreatic cancer cells.PloS one · 2014Article
- Computationally designed peptide inhibitors of the ubiquitin E3 ligase SCF(Fbx4).Chembiochem : a European journal of chemical biology · 2013Article
- Article
- Efficiency of cellular delivery of antisense peptide nucleic acid by electroporation depends on charge and electroporation geometry.Oligonucleotides · 2011Article
- Telomeres, lifestyle, cancer, and aging.Current opinion in clinical nutrition and metabolic care · 2011Review
- Telomere shortening sensitizes cancer cells to selected cytotoxic agents: in vitro and in vivo studies and putative mechanisms.PloS one · 2010Article
- Telomeres and telomerase: from discovery to clinical trials.Chemistry & biology · 2009Article
- Dysfunctional homologous recombination mediates genomic instability and progression in myeloma.Blood · 2009Article
- Methods for assessing DNA hybridization of peptide nucleic acid-titanium dioxide nanoconjugates.Analytical biochemistry · 2008Article
- Telomere maintenance in laser capture microdissection-purified Barrett's adenocarcinoma cells and effect of telomerase inhibition in vivo.Clinical cancer research : an official journal of the American Association for Cancer Research · 2008Article
- Article
- Induction of nitric oxide synthase-dependent telomere shortening after functional inhibition of Hsp90 in human tumor cells.Molecular and cellular biology · 2006Article
- Telomerase inhibition in cancer therapeutics: molecular-based approaches.Current medicinal chemistry · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomerase activity, the ability to add telomeric repeats to the ends of chromosomes, has been detected in most immortal cell lines including tumor cells, but is low or absent in most diploid, mortal cells such as those of somatic tissues. Peptide nucleic acids (PNAs), analogs of DNA or RNA which bind to complementary nucleic acids with very high affinity, were co-electroporated into immortal human cells along with a selectable plasmid. Introduction of PNAs inverse-complementary to telomerase RNA effectively inhibited telomerase activity in intact cells, shortened telomeres, reduced colony size, and arrested cell proliferation after a lag period of 5-30 cell generations, consistent with suppression of their 'immortality'. Electroporation of selection plasmid alone had no effect, while PNAs of altered sequence were markedly less effective in each assay. This constitutes the first demonstration of cell growth arrest through telomerase inhibition, upon treatment of intact cells with an exogenous compound which can be efficiently delivered in vivo. The phenotype of telomerase-inhibited transformed cells differs from senescence of normal diploid fibroblasts, but rather resembles the crisis state of incompletely transformed cells.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.