ArticleImmunology1999
Analysis of immunoglobulin E VH transcripts in a bronchial biopsy of an asthmatic patient confirms bias towards VH5, and indicates local clonal expansion, somatic mutation and isotype switch events.
Article in Immunology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Review
- Peripheral IgE Repertoires of Healthy Donors Carry Moderate Mutation Loads and Do Not Overlap With Other Isotypes.Frontiers in immunology · 2019Article
- IgE repertoire and immunological memory: compartmental regulation and antibody function.International immunology · 2018Review
- Local Clonal Diversification and Dissemination of B Lymphocytes in the Human Bronchial Mucosa.Frontiers in immunology · 2018Article
- Antibodies and superantibodies in patients with chronic rhinosinusitis with nasal polyps.The Journal of allergy and clinical immunology · 2017Article
- The alpha-gal story: lessons learned from connecting the dots.The Journal of allergy and clinical immunology · 2015Review
- Influence of seasonal exposure to grass pollen on local and peripheral blood IgE repertoires in patients with allergic rhinitis.The Journal of allergy and clinical immunology · 2014Article
- A tool kit for rapid cloning and expression of recombinant antibodies.Scientific reports · 2014Article
- Human IgE against the major allergen Bet v 1--defining an epitope with limited cross-reactivity between different PR-10 family proteins.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2014Article
- The human IgE repertoire.International archives of allergy and immunology · 2014Review
- Frequency Patterns of T-Cell Exposed Amino Acid Motifs in Immunoglobulin Heavy Chain Peptides Presented by MHCs.Frontiers in immunology · 2014Review
- IgE-associated IGHV genes from venom and peanut allergic individuals lack mutational evidence of antigen selection.PloS one · 2014Article
- What is the source of serum allergen-specific IgE?Current allergy and asthma reports · 2013Review
- A Temporal Model of Human IgE and IgG Antibody Function.Frontiers in immunology · 2013Article
- Characterization of CD19(+)CD23(+)B2 lymphocytes in the allergic airways of BALB/c mice in response to the inhalation of Aspergillus fumigatus conidia.The open immunology journal · 2012Article
- Mast cell function is not altered by Coronin-1A deficiency.Journal of leukocyte biology · 2010Article
- Article
- Mast cell survival and activation by IgE in the absence of antigen: a consideration of the biologic mechanisms and relevance.Journal of immunology (Baltimore, Md. : 1950) · 2005Review
- Airway generation-specific differences in the spatial distribution of immune cells and cytokines in allergen-challenged rhesus monkeys.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2005Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunoglobulin E (IgE)-dependent mechanisms play a pivotal role in mediating allergic disease. Previously, VH-Cepsilon transcripts from blood or spleen of atopic asthmatics have been analysed for VH gene usage and patterns of somatic mutation. An over-representation of the minor VH5 family has been observed, consistent with a superantigen drive. As local mucosal events in IgE production may be more significant in the disease process, we have analysed VH-Cepsilon transcripts from a bronchial biopsy of a patient with severe asthma. VH5 predominance was confirmed with 10 of 30 unique clones derived from this family. Repeated sequences, some with intraclonal variation, revealed clonal expansion and continuing mutational activity at the site. Unexpectedly, three unmutated VH-Cepsilon sequences were found, indicating that isotype switching to IgE can occur without mutation. Detection of a sister clone with extensive mutations was again consistent with local mutational activity. Evidence for local isotype switching was obtained by identification of clonally related immunoglobulin M (IgM), immunoglobulin G (IgG) and immunoglobulin E (IgE) sequences. However, in contrast to findings in blood, no IgG4 transcripts clonally related to IgE were detected, suggesting that the balance between synthesis of IgG4 and IgE may differ between systemic and local sites. These data confirm a VH5 bias in IgE, and support the concept that IgE-synthesizing B cells arise via local differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.