ArticlePlant physiology1999
Changes in cytosolic pH within Arabidopsis root columella cells play a key role in the early signaling pathway for root gravitropism.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 140 citations in OpenAlex.
- Root pH variation of herbaceous plants among plant functional groups in response to climate and soil gradients on the Tibetan alpine grasslands.Ecology and evolution · 2024Article
- Molecular mechanism of brassinosteroids involved in root gravity response based on transcriptome analysis.BMC plant biology · 2024Article
- Review
- Entanglement of Arabidopsis Seedlings to a Mesh Substrate under Microgravity Conditions in KIBO on the ISS.Plants (Basel, Switzerland) · 2022Article
- The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis.International journal of molecular sciences · 2021Article
- The gravistimulation-induced very slow CaScientific reports · 2021Article
- Distribution of Endogenous NO Regulates Early Gravitropic Response and PIN2 Localization in Arabidopsis Roots.Frontiers in plant science · 2018Article
- A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe.Frontiers in plant science · 2017Article
- Striking the Right Chord: Signaling Enigma during Root Gravitropism.Frontiers in plant science · 2017Review
- Convergence and Divergence of Signaling Events in Guard Cells during Stomatal Closure by Plant Hormones or Microbial Elicitors.Frontiers in plant science · 2016Review
- Morphometric analyses of petioles of seedlings grown in a spaceflight experiment.Journal of plant research · 2015Article
- New insights into root gravitropic signalling.Journal of experimental botany · 2015Review
- Self-incompatibility-induced programmed cell death in field poppy pollen involves dramatic acidification of the incompatible pollen tube cytosol.Plant physiology · 2015Article
- Strategies of seedlings to overcome their sessile nature: auxin in mobility control.Frontiers in plant science · 2015Review
- Arabidopsis Glutathione Transferases U24 and U25 Exhibit a Range of Detoxification Activities with the Environmental Pollutant and Explosive, 2,4,6-Trinitrotoluene.Plant physiology · 2014Article
- Systems analysis of auxin transport in the Arabidopsis root apex.The Plant cell · 2014Article
- Multiple roles for membrane-associated protein trafficking and signaling in gravitropism.Frontiers in plant science · 2012Article
- Article
- Plasticity of Arabidopsis root gravitropism throughout a multidimensional condition space quantified by automated image analysis.Plant physiology · 2010Article
- Joining forces: the interface of gravitropism and plastid protein import.Plant signaling & behavior · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ratiometric wide-field fluorescence microscopy with 1',7'- bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF)-dextran demonstrated that gravistimulation leads to rapid changes in cytoplasmic pH (pHc) in columella cells of Arabidopsis roots. The pHc of unstimulated columella cells in tiers 2 and 3, known sites of graviperception (E.B. Blancaflor, J.B. Fasano, S. Gilroy [1998] Plant Physiol 116: 213-222), was 7.22 +/- 0.02 pH units. Following gravistimulation, the magnitude and direction of pHc changes in these cells depended on their location in the columella. Cells in the lower side of tier 2 became more alkaline by 0.4 unit within 55 s of gravistimulation, whereas alkalinization of the cells on the upper side was slower (100 s). In contrast, all cells in tier 3 acidified by 0.4 pH unit within 480 s after gravistimulation. Disrupting these pHc changes in the columella cells using pHc modifiers at concentrations that do not affect root growth altered the gravitropic response. Acidifying agents, including bafilomycin A1, enhanced curvature, whereas alkalinizing agents disrupted gravitropic bending. These results imply that pHc changes in the gravisensing cells and the resultant pH gradients across the root cap are important at an early stage in the signal cascade leading to the gravitropic response.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.