ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Thrombin-induced growth cone collapse: involvement of phospholipase A(2) and eicosanoid generation.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Mammalian lipoxygenases and their biological relevance.Biochimica et biophysica acta · 2015Review
- Thrombin enhances NGF-mediated neurite extension via increased and sustained activation of p44/42 MAPK and p38 MAPK.PloS one · 2014Article
- Interactions between α-tocopherol, polyunsaturated fatty acids, and lipoxygenases during embryogenesis.Free radical biology & medicine · 2014Review
- Repulsive axon guidance cues ephrin-A2 and slit3 stop protrusion of the growth cone leading margin concurrently with inhibition of ADF/cofilin and ERM proteins.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Group IVA phospholipase A₂ is necessary for growth cone repulsion and collapse.Journal of neurochemistry · 2012Article
- The extended cleavage specificity of human thrombin.PloS one · 2012Article
- Serine proteases, serine protease inhibitors, and protease-activated receptors: roles in synaptic function and behavior.Brain research · 2011Review
- Munc18 and Munc13 regulate early neurite outgrowth.Biology of the cell · 2010Article
- Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding.Molecular biology of the cell · 2006Article
- Contribution of extracellular proteolysis and microglia to intracerebral hemorrhage.Neurocritical care · 2005Review
- Growth cone collapse induced by semaphorin 3A requires 12/15-lipoxygenase.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Growth cone collapse through coincident loss of actin bundles and leading edge actin without actin depolymerization.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The studies presented here explore intracellular signals resulting from the action of repellents on growth cones. Growth cone challenge with thrombin or thrombin receptor-activating peptide (TRAP) triggers collapse via a receptor-mediated process. The results indicate that this involves activation of cytosolic phospholipase A(2) (PLA(2)) and eicosanoid synthesis. The collapse response to repellents targets at least two functional units of the growth cone, the actin cytoskeleton and substratum adhesion sites. We show in a cell-free assay that thrombin and TRAP cause the detachment of isolated growth cones from laminin. Biochemical analyses of isolated growth cones reveal that thrombin and TRAP stimulate cytosolic PLA(2) but not phospholipase C. In addition, thrombin stimulates synthesis of 12- and 15-hydroxyeicosatetraenoic acid (HETE) from the released arachidonic acid via a lipoxygenase (LO) pathway. A selective LO inhibitor blocks 12/15-HETE synthesis in growth cones and inhibits thrombin-induced growth cone collapse. Exogenously applied 12(S)-HETE mimics the thrombin effect and induces growth cone collapse in culture. These observations indicate that thrombin-induced growth cone collapse occurs by a mechanism that involves the activation of cytosolic PLA(2) and the generation of 12/15-HETE.
Indexed as
Identifiers
10594066PMC6784923W2159975206What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.