ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Blue- and green-absorbing visual pigments of Drosophila: ectopic expression and physiological characterization of the R8 photoreceptor cell-specific Rh5 and Rh6 rhodopsins.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed, 249 citations in OpenAlex.
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- Molecular Evolution of Malacostracan Short Wavelength Sensitive Opsins.Journal of molecular evolution · 2023Article
- Different spectral sensitivities of ON- and OFF-motion pathways enhance the detection of approaching color objects in Drosophila.Nature communications · 2023Article
- Nematostella vectensis exemplifies the exceptional expansion and diversity of opsins in the eyeless Hexacorallia.EvoDevo · 2023Article
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- Multi-neuronal recording in unrestrained animals with all acousto-optic random-access line-scanning two-photon microscopy.Frontiers in neuroscience · 2023Article
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- Single cell RNA sequencing of the adult Drosophila eye reveals distinct clusters and novel marker genes for all major cell types.Communications biology · 2022Article
- The diversity of invertebrate visual opsins spanning Protostomia, Deuterostomia, and Cnidaria.Developmental biology · 2022Review
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- The Role of Reversible Phosphorylation ofInternational journal of molecular sciences · 2022Review
- Molecular advances to study the function, evolution and spectral tuning of arthropod visual opsins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
51 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Color discrimination requires the input of different photoreceptor cells that are sensitive to different wavelengths of light. The Drosophila visual system contains multiple classes of photoreceptor cells that differ in anatomical location, synaptic connections, and spectral sensitivity. The Rh5 and Rh6 opsins are expressed in nonoverlapping sets of R8 cells and are the only Drosophila visual pigments that remain uncharacterized. In this study, we ectopically expressed Rh5 and Rh6 in the major class of photoreceptor cells (R1-R6) and show them to be biologically active in their new environment. The expression of either Rh5 or Rh6 in "blind" ninaE(17) mutant flies, which lack the gene encoding the visual pigment of the R1-R6 cells, fully rescues the light response. Electrophysiological analysis showed that the maximal spectral sensitivity of the R1-R6 cells is shifted to 437 or 508 nm when Rh5 or Rh6, respectively, is expressed in these cells. These spectral sensitivities are in excellent agreement with intracellular recordings of the R8p and R8y cells measured in Calliphora and Musca. Spectrophotometric analyses of Rh5 and Rh6 in vivo by microspectrophotometry, and of detergent-extracted pigments in vitro, showed that Rh5 is reversibly photoconverted to a stable metarhodopsin (lambda(max) = 494 nm), whereas Rh6 appears to be photoconverted to a metarhodopsin (lambda(max) = 468 nm) that is less thermally stable. Phylogenetically, Rh5 belongs to a group of short-wavelength-absorbing invertebrate visual pigments, whereas Rh6 is related to a group of long-wavelength-absorbing pigments and is the first member of this class to be functionally characterized.
Indexed as
Identifiers
10594055PMC6784940W1919843837What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.