ArticleMolecular and cellular biology2000
The G-protein beta subunit GPB1 is required for mating and haploid fruiting in Cryptococcus neoformans.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 168 citations in OpenAlex.
- Dissecting G-protein signaling pathways in the fruit pathogen Penicillium expansum: implications for pathogenesis and patulin production.Molecular horticulture · 2026Article
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- Research on the Molecular Interaction Mechanism between Plants and Pathogenic Fungi.International journal of molecular sciences · 2022Review
- Mating Systems in True Morels (Microbiology and molecular biology reviews : MMBR · 2021Review
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- Genetic Transformation inJournal of fungi (Basel, Switzerland) · 2021Review
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- Life Cycle ofAnnual review of microbiology · 2019Review
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- The cAMP/Protein Kinase a Pathway Regulates Virulence and Adaptation to Host Conditions inFrontiers in cellular and infection microbiology · 2019Review
- Pheromone expression reveals putative mechanism of unisexuality in a saprobic ascomycete fungus.PloS one · 2018Article
- Glucosamine stimulates pheromone-independent dimorphic transition in Cryptococcus neoformans by promoting Crz1 nuclear translocation.PLoS genetics · 2017Article
- Expansion of Signal Transduction Pathways in Fungi by Extensive Genome Duplication.Current biology : CB · 2016Article
- Regulatory roles of phosphorylation in model and pathogenic fungi.Medical mycology · 2016Review
- The cAMP/protein kinase A signaling pathway in pathogenic basidiomycete fungi: Connections with iron homeostasis.Journal of microbiology (Seoul, Korea) · 2015Review
- Article
- Crystal structure of Gib2, a signal-transducing protein scaffold associated with ribosomes in Cryptococcus neoformans.Scientific reports · 2015Article
- A Ric8/synembryn homolog promotes Gpa1 and Gpa2 activation to respectively regulate cyclic AMP and pheromone signaling in Cryptococcus neoformans.Eukaryotic cell · 2014Article
- Sexual reproduction of human fungal pathogens.Cold Spring Harbor perspectives in medicine · 2014Review
27 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cryptococcus neoformans is an opportunistic fungal pathogen with a defined sexual cycle. The gene encoding a heterotrimeric G-protein beta subunit, GPB1, was cloned and disrupted. gpb1 mutant strains are sterile, indicating a role for this gene in mating. GPB1 plays an active role in mediating responses to pheromones in early mating steps (conjugation tube formation and cell fusion) and signals via a mitogen-activated protein (MAP) kinase cascade in both MATalpha and MATa cells. The functions of GPB1 are distinct from those of the Galpha protein GPA1, which functions in a nutrient-sensing cyclic AMP (cAMP) pathway required for mating, virulence factor induction, and virulence. gpb1 mutant strains are also defective in monokaryotic fruiting in response to nitrogen starvation. We show that MATa cells stimulate monokaryotic fruiting of MATalpha cells, possibly in response to mating pheromone, which may serve to disperse cells and spores to locate mating partners. In summary, the Gbeta subunit GPB1 and the Galpha subunit GPA1 function in distinct signaling pathways: one (GPB1) senses pheromones and regulates mating and haploid fruiting via a MAP kinase cascade, and the other (GPA1) senses nutrients and regulates mating, virulence factors, and pathogenicity via a cAMP cascade.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.