ArticleMolecular and cellular biology2000
Establishment of distinct MyoD, E2A, and twist DNA binding specificities by different basic region-DNA conformations.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Mutations to transcription factor MAX allosterically increase DNA selectivity by altering folding and binding pathways.Nature communications · 2025Article
- NovelInternational journal of ophthalmology · 2025Article
- Transcriptional Targets of TWIST1 in Human Mesenchymal Stem/Stromal Cells Mechanistically Link Stem/Progenitor and Paracrine Functions.Stem cells (Dayton, Ohio) · 2023Article
- Homodimeric and Heterodimeric Interactions among Vertebrate Basic Helix-Loop-Helix Transcription Factors.International journal of molecular sciences · 2021Review
- Mechanisms of Binding Specificity among bHLH Transcription Factors.International journal of molecular sciences · 2021Review
- Cis-regulatory determinants of MyoD function.Nucleic acids research · 2018Article
- Gamma synuclein is a novel Twist1 target that promotes TGF-β-induced cancer cell migration and invasion.Cell death & disease · 2018Article
- Requirement of the fusogenic micropeptide myomixer for muscle formation in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- MESP1 Mutations in Patients with Congenital Heart Defects.Human mutation · 2016Article
- A Clinical Indications Prediction Scale Based on TWIST1 for Human Mesenchymal Stem Cells.EBioMedicine · 2016Article
- Opposite regulation of MDM2 and MDMX expression in acquisition of mesenchymal phenotype in benign and cancer cells.Oncotarget · 2015Article
- Id4 suppresses MMP2-mediated invasion of glioblastoma-derived cells by direct inactivation of Twist1 function.Oncogene · 2015Article
- N-glycosylation regulates ADAM8 processing and activation.The Journal of biological chemistry · 2014Article
- Myogenic enhancers regulate expression of the facioscapulohumeral muscular dystrophy-associated DUX4 gene.Molecular and cellular biology · 2014Article
- Discrete levels of Twist activity are required to direct distinct cell functions during gastrulation and somatic myogenesis.PloS one · 2014Article
- Analysis of variation at transcription factor binding sites in Drosophila and humans.Genome biology · 2012Article
- Computational modeling of the bHLH domain of the transcription factor TWIST1 and R118C, S144R and K145E mutants.BMC bioinformatics · 2012Article
- Computational modeling on the recognition of the HRE motif by HIF-1: molecular docking and molecular dynamics studies.Journal of molecular modeling · 2012Article
- Drosophila lilliputian is required for proneural gene expression in retinal development.Developmental dynamics : an official publication of the American Association of Anatomists · 2012Article
- Bmps and id2a act upstream of Twist1 to restrict ectomesenchyme potential of the cranial neural crest.PLoS genetics · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basic helix-loop-helix (bHLH) proteins perform a wide variety of biological functions. Most bHLH proteins recognize the consensus DNA sequence CAN NTG (the E-box consensus sequence is underlined) but acquire further functional specificity by preferring distinct internal and flanking bases. In addition, induction of myogenesis by MyoD-related bHLH proteins depends on myogenic basic region (BR) and BR-HLH junction residues that are not essential for binding to a muscle-specific site, implying that their BRs may be involved in other critical interactions. We have investigated whether the myogenic residues influence DNA sequence recognition and how MyoD, Twist, and their E2A partner proteins prefer distinct CAN NTG sites. In MyoD, the myogenic BR residues establish specificity for particular CAN NTG sites indirectly, by influencing the conformation through which the BR helix binds DNA. An analysis of DNA binding by BR and junction mutants suggests that an appropriate BR-DNA conformation is necessary but not sufficient for myogenesis, supporting the model that additional interactions with this region are important. The sequence specificities of E2A and Twist proteins require the corresponding BR residues. In addition, mechanisms that position the BR allow E2A to prefer distinct half-sites as a heterodimer with MyoD or Twist, indicating that the E2A BR can be directed toward different targets by dimerization with different partners. Our findings indicate that E2A and its partner bHLH proteins bind to CAN NTG sites by adopting particular preferred BR-DNA conformations, from which they derive differences in sequence recognition that can be important for functional specificity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.