ArticleMolecular and cellular biology2000
Histone H1 is dispensable for methylation-associated gene silencing in Ascobolus immersus and essential for long life span.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 92 citations in OpenAlex.
- Neofunctionalization of H1 linker histones drives divergent gene expression and histone methylation in Cryptococcus neoformans.Communications biology · 2026Article
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- Histone H1 Limits DNA Methylation in Neurospora crassa.G3 (Bethesda, Md.) · 2016Article
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- Linker Histone H1.2 cooperates with Cul4A and PAF1 to drive H4K31 ubiquitylation-mediated transactivation.Cell reports · 2013Article
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- Geographic patterns of histone H1 encoding genes allelic variation in Aegilops tauschii Coss. (Poaceae).Molecular biology reports · 2012Article
- DNA methyltransferase 3b preferentially associates with condensed chromatin.Nucleic acids research · 2011Article
- Structure of the H1 C-terminal domain and function in chromatin condensation.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2011Review
- PARP is involved in replicative aging in Neurospora crassa.Fungal genetics and biology : FG & B · 2010Article
- Epigenome manipulation as a pathway to new natural product scaffolds and their congeners.Natural product reports · 2010Review
- Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins.The Journal of biological chemistry · 2009Article
- Depletion of human histone H1 variants uncovers specific roles in gene expression and cell growth.PLoS genetics · 2008Article
- Histone modifications and chromatin dynamics: a focus on filamentous fungi.FEMS microbiology reviews · 2008Review
- Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription.The Journal of biological chemistry · 2008Article
- Drosophila ribosomal proteins are associated with linker histone H1 and suppress gene transcription.Genes & development · 2006Article
- The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in Tetrahymena thermophila.Molecular and cellular biology · 2005Article
- Suppression of histone H1 genes in Arabidopsis results in heritable developmental defects and stochastic changes in DNA methylation.Genetics · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A gene encoding a protein that shows sequence similarity with the histone H1 family only was cloned in Ascobolus immersus. The deduced peptide sequence presents the characteristic three-domain structure of metazoan linker histones, with a central globular region, an N-terminal tail, and a long positively charged C-terminal tail. By constructing an artificial duplication of this gene, named H1, it was possible to methylate and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing, underwent MIP, and showed the same methylation-associated chromatin modifications as did wild-type strains. However, they displayed an increased accessibility of micrococcal nuclease to chromatin, whether DNA was methylated or not, and exhibited a hypermethylation of the methylated genome compartment. These features are taken to imply that Ascobolus H1 histone is a ubiquitous component of chromatin which plays no role in methylation-associated gene silencing. Mutant strains lacking histone H1 reproduced normally through sexual crosses and displayed normal early vegetative growth. However, between 6 and 13 days after germination, they abruptly and consistently stopped growing, indicating that Ascobolus H1 histone is necessary for long life span. This constitutes the first observation of a physiologically important phenotype associated with the loss of H1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.