ArticleThe Plant cell1999
Analysis of flanking sequences from dissociation insertion lines: a database for reverse genetics in Arabidopsis.
Article in The Plant cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers, 1 of them a synthesis that pooled it.
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Who cites it
124 citing papers in PubMed, 1 synthesis or guideline pooled it, 310 citations in OpenAlex.
- Meta-analyses of microarrays of Arabidopsis asymmetric leaves1 (as1), as2 and their modifying mutants reveal a critical role for the ETT pathway in stabilization of adaxial-abaxial patterning and cell division during leaf development.Plant & cell physiology · 2013Pooled it
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- Next Generation Sequencing Based Forward Genetic Approaches for Identification and Mapping of Causal Mutations in Crop Plants: A Comprehensive Review.Plants (Basel, Switzerland) · 2020Review
- Virus-Induced Gene Silencing (VIGS) in Aegilops tauschii and Its Use in Functional Analysis of AetDREB2.Molecular biotechnology · 2018Article
- A collection of enhancer trap insertional mutants for functional genomics in tomato.Plant biotechnology journal · 2017Article
- ThePeerJ · 2017Article
- RALFL34 regulates formative cell divisions in Arabidopsis pericycle during lateral root initiation.Journal of experimental botany · 2016Article
- Recombination Rate Heterogeneity within Arabidopsis Disease Resistance Genes.PLoS genetics · 2016Article
- Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores.The Plant cell · 2016Article
- AINTEGUMENTA and the D-type cyclin CYCD3;1 regulate root secondary growth and respond to cytokinins.Biology open · 2015Article
- A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNAIle(GAU).Plant physiology · 2015Article
- Frequent problems and their resolutions by using thermal asymmetric interlaced PCR (TAIL-PCR) to clone genes inBiotechnology, biotechnological equipment · 2015Article
- Phenotype to genotype using forward-genetic Mu-seq for identification and functional classification of maize mutants.Frontiers in plant science · 2014Article
- Genetic analysis of ectopic growth suppression during planar growth of integuments mediated by the Arabidopsis AGC protein kinase UNICORN.BMC plant biology · 2013Article
- Arabidopsis light-dependent protochlorophyllide oxidoreductase A (PORA) is essential for normal plant growth and development.Plant molecular biology · 2012Article
- Mutagenesis of barley malting quality QTLs with Ds transposons.Functional & integrative genomics · 2012Article
- Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery.BMC genomics · 2012Article
- CRYPTIC PRECOCIOUS/MED12 is a novel flowering regulator with multiple target steps in Arabidopsis.Plant & cell physiology · 2012Article
64 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have generated Dissociation (Ds) element insertions throughout the Arabidopsis genome as a means of random mutagenesis. Here, we present the molecular analysis of genomic sequences that flank the Ds insertions of 931 independent transposant lines. Flanking sequences from 511 lines proved to be identical or homologous to DNA or protein sequences in public databases, and disruptions within known or putative genes were indicated for 354 lines. Because a significant portion (45%) of the insertions occurred within sequences defined by GenBank BAC and P1 clones, we were able to assess the distribution of Ds insertions throughout the genome. We discovered a significant preference for Ds transposition to the regions adjacent to nucleolus organizer regions on chromosomes 2 and 4. Otherwise, the mapped insertions appeared to be evenly dispersed throughout the genome. For any given gene, insertions preferentially occurred at the 5' end, although disruption was clearly possible at any intragenic position. The insertion sites of >500 lines that could be characterized by reference to public databases are presented in a tabular format at http://www.plantcell. org/cgi/content/full/11/12/2263/DC1. This database should be of value to researchers using reverse genetics approaches to determine gene function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.