ArticleJournal of virology2000
A heterologous, high-affinity RNA ligand for human immunodeficiency virus Gag protein has RNA packaging activity.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- HIV Genome-Wide Protein Associations: a Review of 30 Years of Research.Microbiology and molecular biology reviews : MMBR · 2016Pooled it
- Article
- Initiation of HIV-1 Gag lattice assembly is required for recognition of the viral genome packaging signal.eLife · 2023Article
- Rational design of self-assembled RNA nanostructures for HIV-1 virus assembly blockade.Nucleic acids research · 2022Article
- Article
- Potential inhibition of HIV-1 encapsidation by oligoribonucleotide-dendrimer nanoparticle complexes.International journal of nanomedicine · 2017Article
- Retrospective on the all-in-one retroviral nucleocapsid protein.Virus research · 2014Review
- Deciphering the role of the Gag-Pol ribosomal frameshift signal in HIV-1 RNA genome packaging.Journal of virology · 2014Article
- The determination of importance of sequences neighboring the Psi sequence in lentiviral vector transduction and packaging efficiency.PloS one · 2012Article
- Beyond plasma membrane targeting: role of the MA domain of Gag in retroviral genome encapsidation.Journal of molecular biology · 2011Review
- Structural determinants and mechanism of HIV-1 genome packaging.Journal of molecular biology · 2011Review
- Mutations in matrix and SP1 repair the packaging specificity of a Human Immunodeficiency Virus Type 1 mutant by reducing the association of Gag with spliced viral RNA.Retrovirology · 2010Article
- The remarkable frequency of human immunodeficiency virus type 1 genetic recombination.Microbiology and molecular biology reviews : MMBR · 2009Review
- Analysis of human immunodeficiency virus type 1 vector cis- and trans-acting elements production by means of Semliki Forest virus.Gene therapy · 2009Article
- HIV-2 RNA dimerization is regulated by intramolecular interactions in vitro.RNA (New York, N.Y.) · 2007Article
- RNA structure and packaging signals in the 5' leader region of the human immunodeficiency virus type 1 genome.Journal of virology · 2002Article
- Functional analysis of the murine sarcoma virus RNA packaging sequence.Journal of virology · 2002Article
- Characterization of Rous sarcoma virus Gag particles assembled in vitro.Journal of virology · 2001Article
- Article
- Sequences in the 5' and 3' R elements of human immunodeficiency virus type 1 critical for efficient reverse transcription.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Retroviral RNA encapsidation depends on the specific binding of Gag proteins to packaging (psi) signals in genomic RNA. We investigated whether an in vitro-selected, high-affinity RNA ligand for the nucleocapsid (NC) portion of the Gag protein from human immunodeficiency virus type 1 (HIV-1) could mediate packaging into HIV-1 virions. We find that this ligand can functionally substitute for one of the Gag-binding elements (termed SL3) in the HIV-1 psi locus to support packaging and viral infectivity in cis. By contrast, this ligand, which fails to dimerize spontaneously in vitro, is unable to replace a different psi element (termed SL1) which is required for both Gag binding and dimerization of the HIV-1 genome. A single point mutation within the ligand that eliminates high-affinity in vitro Gag binding also abolishes its packaging activity at the SL3 position. These results demonstrate that specific binding of Gag or NC protein is a critical determinant of genomic RNA packaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.