ArticleJournal of virology2000
Extended analysis of the in vitro tropism of porcine endogenous retrovirus.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
67 citing papers in PubMed, 208 citations in OpenAlex.
- The Transmembrane Envelope Protein of Porcine Endogenous Retroviruses Modulates Cytokine Release and Gene Expression in Human Immune Cells.International journal of molecular sciences · 2026Article
- Co-Cultivation Assays for Detecting Infectious Human-Tropic Porcine Endogenous Retroviruses (PERVs).International journal of molecular sciences · 2025Review
- Surveillance and prevention of infection in clinical xenotransplantation.Clinical microbiology reviews · 2025Review
- Analysis of PERV-C superinfection resistance using HA-tagged viruses.Retrovirology · 2023Article
- Isolation of an Ecotropic Porcine Endogenous Retrovirus PERV-C from a Yucatan SLAJournal of virology · 2023Article
- Porcine endogenous retrovirus: classification, molecular structure, regulation, function, and potential risk in xenotransplantation.Functional & integrative genomics · 2023Review
- Scan of the endogenous retrovirus sequences across the swine genome and survey of their copy number variation and sequence diversity among various Chinese and Western pig breeds.Zoological research · 2022Article
- Rare isolation of human-tropic recombinant porcine endogenous retroviruses PERV-A/C from Göttingen minipigs.Virology journal · 2022Article
- Cardiac xenotransplantation: a promising way to treat advanced heart failure.Heart failure reviews · 2022Review
- High Prevalence of Recombinant Porcine Endogenous Retroviruses (PERV-A/Cs) in Minipigs: A Review on Origin and Presence.Viruses · 2021Review
- The origin of porcine endogenous retroviruses (PERVs).Archives of virology · 2021Review
- A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies.Scientific reports · 2020Article
- Article
- Infection in xenotransplantation: opportunities and challenges.Current opinion in organ transplantation · 2019Review
- Why was PERV not transmitted during preclinical and clinical xenotransplantation trials and after inoculation of animals?Retrovirology · 2018Review
- A comprehensive microbiological safety approach for agarose encapsulated porcine islets intended for clinical trials.Xenotransplantation · 2016Article
- Susceptibility of porcine endogenous retrovirus to anti-retroviral inhibitors.Xenotransplantation · 2016Article
- Immune modulation in xenotransplantation.Archivum immunologiae et therapiae experimentalis · 2015Review
- Identification of Porcine Endogenous Retrovirus (PERV) packaging sequence and development of PERV packaging viral vector system.Journal of microbiology (Seoul, Korea) · 2015Article
- Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that mitogenic activation of porcine peripheral blood mononuclear cells resulted in production of porcine endogenous retrovirus(es) (PERV[s]) capable of productively infecting human cells (C. Wilson et al., J. Virol. 72:3082-3087, 1998). We now extend that analysis to show that additional passage of isolated virus, named here PERV-NIH, through a human cell line yielded a viral population with a higher titer of infectious virus on human cells than the initial isolate. We show that in a single additional passage on a human cell line, the increase in infectivity for human cells is accounted for by selection against variants carrying pig-tropic envelope sequences (PERV-C) as well as by enrichment for replication-competent genomes. Sequence analysis of the envelope cDNA present in virions demonstrated that the envelope sequence of PERV-NIH is related to but distinct from previously reported PERV envelopes. The in vitro host range of PERV was studied in human primary cells and cell lines, as well as in cell lines from nonhuman primate and other species. This analysis reveals three patterns of susceptibility to infection among these host cells: (i) cells are resistant to infection in our assay; (ii) cells are infected by virus, as viral RNA is detected in the supernatant by reverse transcription-PCR, but the cells are not permissive to productive replication and spread; and (iii) cells are permissive to low-level productive replication. Certain cell lines were permissive for efficient productive infection and spread. These results may prove useful in designing appropriate animal models to assess the in vivo infectivity properties of PERV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.