ArticleProceedings of the National Academy of Sciences of the United States of America1999
Voltage-induced membrane displacement in patch pipettes activates mechanosensitive channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Ectopic mouse TMC1 and TMC2 alone form mechanosensitive channels that are potently modulated by TMIE.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanosensitive potassium channels in neurons projecting cardiac axons of the nodose ganglion in rats.Frontiers in physiology · 2025Article
- Ultrasound Mediated Cellular Deflection Results in Cellular Depolarization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Mechanosensitive gating of Kv channels.PloS one · 2015Review
- Gd3+ and calcium sensitive, sodium leak currents are features of weak membrane-glass seals in patch clamp recordings.PloS one · 2014Article
- Toward understanding protocell mechanosensation.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2011Review
- Whole-Cell Electrical Activity Under Direct Mechanical Stimulus by AFM Cantilever Using Planar Patch Clamp Chip Approach.Cellular and molecular bioengineering · 2011Article
- Mechanosensitivity of Nav1.5, a voltage-sensitive sodium channel.The Journal of physiology · 2010Article
- Biophysics and structure of the patch and the gigaseal.Biophysical journal · 2009Article
- Nav channel mechanosensitivity: activation and inactivation accelerate reversibly with stretch.Biophysical journal · 2007Article
- Ionic requirements for membrane-glass adhesion and giga seal formation in patch-clamp recording.Biophysical journal · 2007Article
- Mechanosensitive channel properties and membrane mechanics in mouse dystrophic myotubes.The Journal of physiology · 2007Article
- Ion conduction through MscS as determined by electrophysiology and simulation.Biophysical journal · 2007Article
- Cooperative gating between single HCN pacemaker channels.The Journal of general physiology · 2006Article
- Changes in mechanosensitive channel gating following mechanical stimulation in skeletal muscle myotubes from the mdx mouse.The Journal of physiology · 2002Article
- Two-dimensional kinetic analysis suggests nonsequential gating of mechanosensitive channels in Xenopus oocytes.Biophysical journal · 2001Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The patch-clamp technique allows currents to be recorded through single ion channels in patches of cell membrane in the tips of glass pipettes. When recording, voltage is typically applied across the membrane patch to drive ions through open channels and to probe the voltage-sensitivity of channel activity. In this study, we used video microscopy and single-channel recording to show that prolonged depolarization of a membrane patch in borosilicate pipettes results in delayed slow displacement of the membrane into the pipette and that this displacement is associated with the activation of mechanosensitive (MS) channels in the same patch. The membrane displacement, approximately 1 micrometer with each prolonged depolarization, occurs after variable delays ranging from tens of milliseconds to many seconds and is correlated in time with activation of MS channels. Increasing the voltage step shortens both the delay to membrane displacement and the delay to activation. Preventing depolarization-induced membrane displacement by applying positive pressure to the shank of the pipette or by coating the tips of the borosilicate pipettes with soft glass prevents the depolarization-induced activation of MS channels. The correlation between depolarization-induced membrane displacement and activation of MS channels indicates that the membrane displacement is associated with sufficient membrane tension to activate MS channels. Because membrane tension can modulate the activity of various ligand and voltage-activated ion channels as well as some transporters, an apparent voltage dependence of a channel or transporter in a membrane patch in a borosilicate pipette may result from voltage-induced tension rather than from direct modulation by voltage.
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