ArticleMolecular biology of the cell1999
Modulation of endocytic traffic in polarized Madin-Darby canine kidney cells by the small GTPase RhoA.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- Review
- Macrophage/monocyte-specific deletion of Ras homolog gene family member A (RhoA) downregulates fractalkine receptor and inhibits chronic rejection of mouse cardiac allografts.The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · 2017Article
- Cytoskeletal proteins in cortical development and disease: actin associated proteins in periventricular heterotopia.Frontiers in cellular neuroscience · 2015Review
- TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells.Molecular biology of the cell · 2014Article
- Rho GTPases, phosphoinositides, and actin: a tripartite framework for efficient vesicular trafficking.Small GTPases · 2014Review
- Article
- GTP-binding protein-like domain of AGAP1 is protein binding site that allosterically regulates ArfGAP protein catalytic activity.The Journal of biological chemistry · 2012Article
- Concordance and interaction of guanine nucleotide dissociation inhibitor (RhoGDI) with RhoA in oogenesis and early development of the sea urchin.Development, growth & differentiation · 2011Article
- Xenopus Kazrin interacts with ARVCF-catenin, spectrin and p190B RhoGAP, and modulates RhoA activity and epithelial integrity.Journal of cell science · 2010Article
- Cdc42 and the phosphatidylinositol 3-kinase-Akt pathway are essential for PspC-mediated internalization of pneumococci by respiratory epithelial cells.The Journal of biological chemistry · 2009Article
- Rab14 regulates apical targeting in polarized epithelial cells.Traffic (Copenhagen, Denmark) · 2008Article
- Differentiation restricted endocytosis of cell penetrating peptides in MDCK cells corresponds with activities of Rho-GTPases.Pharmaceutical research · 2007Article
- Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 2006Article
- Role of small GTPases in Trypanosoma cruzi invasion in MDCK cell lines.Parasitology research · 2005Article
- Rho1 regulates signaling events required for proper Drosophila embryonic development.Developmental biology · 2005Article
- Rho GTPases and leucocyte-induced endothelial remodelling.The Biochemical journal · 2005Review
- Bidirectional transepithelial IgG transport by a strongly polarized basolateral membrane Fcgamma-receptor.Molecular biology of the cell · 2004Article
- Epithelial cell polarity alters Rho-GTPase responses to Pseudomonas aeruginosa.Molecular biology of the cell · 2004Article
- Functional reconstitution of human FcRn in Madin-Darby canine kidney cells requires co-expressed human beta 2-microglobulin.The Journal of biological chemistry · 2002Article
- Na,K-ATPase activity is required for formation of tight junctions, desmosomes, and induction of polarity in epithelial cells.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Efficient postendocytic membrane traffic in polarized epithelial cells is thought to be regulated in part by the actin cytoskeleton. RhoA modulates assemblies of actin in the cell, and it has been shown to regulate pinocytosis and phagocytosis; however, its effects on postendocytic traffic are largely unexplored. To this end, we expressed wild-type RhoA (RhoAWT), dominant active RhoA (RhoAV14), and dominant inactive RhoA (RhoAN19) in Madin-Darby canine kidney (MDCK) cells expressing the polymeric immunoglobulin receptor. RhoAV14 expression stimulated the rate of apical and basolateral endocytosis, whereas RhoAN19 expression decreased the rate from both membrane domains. Polarized basolateral recycling of transferrin was disrupted in RhoAV14-expressing cells as a result of increased ligand release at the apical pole of the cell. Degradation of basolaterally internalized epidermal growth factor was slowed in RhoAV14-expressing cells. Although apical recycling of immunoglobulin A (IgA) was largely unaffected in cells expressing RhoAV14, transcytosis of basolaterally internalized IgA was severely impaired. Morphological and biochemical analyses demonstrated that a large proportion of IgA internalized from the basolateral pole of RhoAV14-expressing cells remained within basolateral early endosomes and was slow to exit these compartments. RhoAN19 and RhoAWT expression had little effect on these postendocytic pathways. These results indicate that in polarized MDCK cells activated RhoA may modulate endocytosis from both membrane domains and postendocytic traffic at the basolateral pole of the cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.