ArticleMolecular biology of the cell1999
Class VI unconventional myosin is required for spermatogenesis in Drosophila.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 108 citations in OpenAlex.
- Myosin VI Is Associated With the Endoplasmic Reticulum in Regions of Sertoli Cells Containing Tubulobulbar Complexes.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Myosin VI is expressed in developing ovarian follicles inFrontiers in cell and developmental biology · 2025Article
- Expression Analysis in a Dispersal-Fecundity Polyphenism Identifies Growth Regulators and Effectors.Integrative and comparative biology · 2022Article
- PFTAIRE Kinase L63 Interactor 1A (Pif1A Protein) Is Required for Actin Cone Movement during Spermatid Individualization inInternational journal of molecular sciences · 2022Article
- Unconventional translation initiation factor EIF2A is required for Drosophila spermatogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Diverse functions of myosin VI in spermiogenesis.Histochemistry and cell biology · 2021Review
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Spermatogenesis in haploid males of the jewel wasp Nasonia vitripennis.Scientific reports · 2019Article
- Distinct roles of two myosins in C. elegans spermatid differentiation.PLoS biology · 2019Article
- Drosophila Pif1A is essential for spermatogenesis and is the homolog of human CCDC157, a gene associated with idiopathic NOA.Cell death & disease · 2019Article
- Critical roles of long noncoding RNAs in Drosophila spermatogenesis.Genome research · 2016Article
- Diverse functions of myosin VI elucidated by an isoform-specific α-helix domain.Nature structural & molecular biology · 2016Article
- Double-sieving-defective aminoacyl-tRNA synthetase causes protein mistranslation and affects cellular physiology and development.Nature communications · 2014Article
- Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules.Biology open · 2014Article
- Gudu, an Armadillo repeat-containing protein, is required for spermatogenesis in Drosophila.Gene · 2013Article
- The cortical acto-Myosin network: from diffusion barrier to functional gateway in the transport of neurosecretory vesicles to the plasma membrane.Frontiers in endocrinology · 2013Review
- Structure of androcam supports specialized interactions with myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Drosophila spermiogenesis: Big things come from little packages.Spermatogenesis · 2012Article
- Article
- Tissue, cell type and stage-specific ectopic gene expression and RNAi induction in the Drosophila testis.Spermatogenesis · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We have identified partial loss of function mutations in class VI unconventional myosin, 95F myosin, which results in male sterility. During spermatogenesis the germ line precursor cells undergo mitosis and meiosis to form a bundle of 64 spermatids. The spermatids remain interconnected by cytoplasmic bridges until individualization. The process of individualization involves the formation of a complex of cytoskeletal proteins and membrane, the individualization complex (IC), around the spermatid nuclei. This complex traverses the length of each spermatid resolving the shared membrane into a single membrane enclosing each spermatid. We have determined that 95F myosin is a component of the IC whose function is essential for individualization. In wild-type testes, 95F myosin localizes to the leading edge of the IC. Two independent mutations in 95F myosin reduce the amount of 95F myosin in only a subset of tissues, including the testes. This reduction of 95F myosin causes male sterility as a result of defects in spermatid individualization. Germ line transformation with the 95F myosin heavy chain cDNA rescues the male sterility phenotype. IC movement is aberrant in these 95F myosin mutants, indicating a critical role for 95F myosin in IC movement. This report is the first identification of a component of the IC other than actin. We propose that 95F myosin is a motor that participates in membrane reorganization during individualization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.