ArticleMolecular biology of the cell1999
The yeast GRD20 gene is required for protein sorting in the trans-Golgi network/endosomal system and for polarization of the actin cytoskeleton.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 70 citations in OpenAlex.
- COG Complex in Golgi Trafficking and Glycosylation.Sub-cellular biochemistry · 2026Review
- Killer toxin K28 resistance in yeast relies on COG complex-mediated trafficking of the defence factor Ktd1.Journal of cell science · 2025Article
- Maintaining order: COG complex controls Golgi trafficking, processing, and sorting.FEBS letters · 2019Review
- Highly parallel genome variant engineering with CRISPR-Cas9.Nature genetics · 2018Article
- Target silencing of components of the conserved oligomeric Golgi complex impairs HIV-1 replication.Virus research · 2014Article
- Regulating the large Sec7 ARF guanine nucleotide exchange factors: the when, where and how of activation.Cellular and molecular life sciences : CMLS · 2014Review
- Actin acting at the Golgi.Histochemistry and cell biology · 2013Review
- Chlamydia trachomatis hijacks intra-Golgi COG complex-dependent vesicle trafficking pathway.Cellular microbiology · 2012Article
- The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.The Journal of cell biology · 2011Article
- The Caenorhabditis elegans GARP complex contains the conserved Vps51 subunit and is required to maintain lysosomal morphology.Molecular biology of the cell · 2011Article
- pH-dependent cargo sorting from the Golgi.The Journal of biological chemistry · 2011Article
- The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy.The Journal of cell biology · 2010Article
- Role of vesicle tethering factors in the ER-Golgi membrane traffic.FEBS letters · 2009Review
- Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry.Molecular biology of the cell · 2008Article
- Structural features of vps35p involved in interaction with other subunits of the retromer complex.Traffic (Copenhagen, Denmark) · 2007Article
- Dominant-negative behavior of mammalian Vps35 in yeast requires a conserved PRLYL motif involved in retromer assembly.Traffic (Copenhagen, Denmark) · 2007Article
- Avl9p, a member of a novel protein superfamily, functions in the late secretory pathway.Molecular biology of the cell · 2007Article
- Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway.Molecular biology of the cell · 2007Article
- The clathrin adaptor complex 1 directly binds to a sorting signal in Ste13p to reduce the rate of its trafficking to the late endosome of yeast.The Journal of cell biology · 2006Article
- Mutants in trs120 disrupt traffic from the early endosome to the late Golgi.The Journal of cell biology · 2005Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The proper localization of resident membrane proteins to the trans-Golgi network (TGN) involves mechanisms for both TGN retention and retrieval from post-TGN compartments. In this study we report identification of a new gene, GRD20, involved in protein sorting in the TGN/endosomal system of Saccharomyces cerevisiae. A strain carrying a transposon insertion allele of GRD20 exhibited rapid vacuolar degradation of the resident TGN endoprotease Kex2p and aberrantly secreted approximately 50% of the soluble vacuolar hydrolase carboxypeptidase Y. The Kex2p mislocalization and carboxypeptidase Y missorting phenotypes were exhibited rapidly after loss of Grd20p function in grd20 temperature-sensitive mutant strains, indicating that Grd20p plays a direct role in these processes. Surprisingly, little if any vacuolar degradation was observed for the TGN membrane proteins A-ALP and Vps10p, underscoring a difference in trafficking patterns for these proteins compared with that of Kex2p. A grd20 null mutant strain exhibited extremely slow growth and a defect in polarization of the actin cytoskeleton, and these two phenotypes were invariably linked in a collection of randomly mutagenized grd20 alleles. GRD20 encodes a hydrophilic protein that partially associates with the TGN. The discovery of GRD20 suggests a link between the cytoskeleton and function of the yeast TGN.
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