ArticleMolecular biology of the cell1999
Synaptic vesicles form by budding from tubular extensions of sorting endosomes in PC12 cells.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- Assessing the Potential of NGF-Differentiated PC12 Cells as a Model for Synaptic Transmission.Molecular neurobiology · 2025Article
- Drosophila transmembrane protein 214 (dTMEM214) regulates midgut glucose uptake and systemic glucose homeostasis.Developmental biology · 2023Article
- Integrating intracellular nanovesicles into integrin trafficking pathways and beyond.Cellular and molecular life sciences : CMLS · 2022Review
- Taming the Triskelion: Bacterial Manipulation of Clathrin.Microbiology and molecular biology reviews : MMBR · 2019Review
- Disruption of the Man-6-P targeting pathway in mice impairs osteoclast secretory lysosome biogenesis.Traffic (Copenhagen, Denmark) · 2011Article
- Endosomal recycling regulates Anthrax Toxin Receptor 1/Tumor Endothelial Marker 8-dependent cell spreading.Experimental cell research · 2010Article
- APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Roles of BLOC-1 and adaptor protein-3 complexes in cargo sorting to synaptic vesicles.Molecular biology of the cell · 2009Article
- Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2.The Biochemical journal · 2007Article
- A second SNARE role for exocytic SNAP25 in endosome fusion.Molecular biology of the cell · 2006Article
- Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
- Applications of an efficient method for comparing immunogold labelling patterns in the same sets of compartments in different groups of cells.Histochemistry and cell biology · 2004Article
- Biochemical characterization of the coating mechanism of the endosomal donor compartment of synaptic vesicles.Neurochemical research · 2004Article
- Entry of newly synthesized GLUT4 into the insulin-responsive storage compartment is GGA dependent.The EMBO journal · 2004Article
- The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.Molecular biology of the cell · 2004Article
- Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes.The EMBO journal · 2003Article
- Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release.The Journal of cell biology · 2003Article
- Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Rate and extent of phagocytosis in macrophages lacking vamp3.Journal of leukocyte biology · 2002Article
- Bilayered clathrin coats on endosomal vacuoles are involved in protein sorting toward lysosomes.Molecular biology of the cell · 2002Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The putative role of sorting early endosomes (EEs) in synaptic-like microvesicle (SLMV) formation in the neuroendocrine PC12 cell line was investigated by quantitative immunoelectron microscopy. By BSA-gold internalization kinetics, four distinct endosomal subcompartments were distinguished: primary endocytic vesicles, EEs, late endosomes, and lysosomes. As in other cells, EEs consisted of vacuolar and tubulovesicular subdomains. The SLMV marker proteins synaptophysin and vesicle-associated membrane protein 2 (VAMP-2) localized to both the EE vacuoles and associated tubulovesicles. Quantitative analysis showed that the transferrin receptor and SLMV proteins colocalized to a significantly higher degree in primary endocytic vesicles then in EE-associated tubulovesicles. By incubating PC12 cells expressing T antigen-tagged VAMP (VAMP-TAg) with antibodies against the luminal TAg, the recycling pathway of SLMV proteins was directly visualized. At 15 degrees C, internalized VAMP-TAg accumulated in the vacuolar domain of EEs. Upon rewarming to 37 degrees C, the labeling shifted to the tubular part of EEs and to newly formed SLMVs. Our data delineate a pathway in which SLMV proteins together with transferrin receptor are delivered to EEs, where they are sorted into SLMVs and recycling vesicles, respectively.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.