Evidence map›Paper›PMID 10587524›Full record

ArticleThe Journal of clinical investigation1999

Mechanical strain activates BNP gene transcription through a p38/NF-kappaB-dependent mechanism.

F Liang, D G Gardner

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of clinical investigation, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 217 citations in OpenAlex.

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  6. Markers of imminent myocardial infarction.Nature cardiovascular research · 2024
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4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

F LiangMetabolic Research Unit, Department of Medicine, University of California-San Francisco, San Francisco, California 94143, USA.
D G Gardner
University of California, San Francisco · US

Funding

EXPRESSION AND REGULATION OF ATRIAL NATRIURETIC FACTORR01HL035753 · NHLBI · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI GARDNER, DAVID G · 1985 to 2002
$996k
NHLBI NIH HHS HL-35753NHLBI NIH HHS R01 HL035753
6 · The paper itself

Abstract

Application of mechanical strain to neonatal rat ventricular myocytes in culture evokes changes in gene expression reminiscent of those that occur with hypertrophy in vivo, such as stimulation of brain natriuretic peptide (BNP) gene expression. Here, we show that a major component of strain-dependent BNP promoter activation results from stimulation of p38 mitogen-activated protein kinase (MAPK) in the cardiac myocyte. Strain increased p38 activity in a time-dependent fashion. The p38 inhibitor SB203580 led to a reduction of approximately 60% in strain-activated human BNP (hBNP) promoter activity. Cotransfection of wild-type p38 increased both basal and strain-dependent promoter activity, while cotransfection with MKK6AL, a dominant-negative inhibitor of p38 MAPK kinase, resulted in partial inhibition of either p38- or strain-activated hBNP promoter activity. p38 MAPK increased hBNP promoter activity through activation of the transcription factor NF-kappaB. Activation of the hBNP promoter by either p38 or strain was mediated by DNA elements present in the 5' flanking sequence of the gene. Mechanical strain promoted assembly of NF-kappaB components on these DNA elements in vitro. Thus, induction of the hBNP promoter by mechanical strain depends, at least in part, on stimulation of p38 and subsequent activation of NF-kappaB. This activation may play an important role in signaling the increased BNP gene expression that accompanies hemodynamic overload and cardiac hypertrophy in vivo.

Indexed as

I-kappa B ProteinsAnimalsCardiomegalyCells, CulturedDNA-Binding ProteinsEnzyme ActivationEnzyme InhibitorsGenes, ReporterHumansImidazolesMitogen-Activated Protein KinasesMutationMyocardiumNatriuretic Peptide, BrainNF-kappa BNF-KappaB Inhibitor alphaDNA-Binding ProteinsEnzyme InhibitorsI-kappa B ProteinsImidazolesMitogen-Activated Protein KinasesNatriuretic Peptide, BrainNF-kappa BNF-KappaB Inhibitor alphaNFKBIA protein, humanNfkbia protein, ratp38 Mitogen-Activated Protein KinasesPyridinesSB 203580

Identifiers

PMID10587524
PMCPMC409860
OpenAlexW2026472826

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.