ArticleVeterinary immunology and immunopathology1999
Quantitative real-time PCR for the measurement of feline cytokine mRNA.
Article in Veterinary immunology and immunopathology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 238 citations in OpenAlex.
- Evaluation of a novel immunotherapy for treatment of chronic rhinitis in cats.Journal of feline medicine and surgery · 2006Trial
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- First report of visceral Leishmaniasis in abandoned dogs of the urban area in the central region of Colombia.Veterinary research communications · 2025Article
- Selected Pathway Analyses to Gain Mechanistic Insights into the Pathogenesis of Feline Hypertrophic Cardiomyopathy.International journal of molecular sciences · 2025Article
- Antiviral Cellulose-Lignin Assembly Entirely Composed of Plant Cell Wall Components.Applied biochemistry and biotechnology · 2025Article
- Poxviruses in dogs and cats: a genuine threat?Brazilian journal of veterinary medicine · 2025Article
- Comparison of Antiviral Immune Responses in Healthy Cats Induced by Two Immune Therapeutics.Pathogens (Basel, Switzerland) · 2024Article
- Molecular diversity of the VP2 ofJournal of veterinary science · 2022Article
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- Transcriptional inhibition of feline immunodeficiency virus by alpha-amanitin.The Journal of veterinary medical science · 2021Article
- Molecular diversity ofHeliyon · 2020Article
- Genomic and phenotypic analyses of six offspring of a genome-edited hornless bull.Nature biotechnology · 2020Article
- Feline Infectious Peritonitis as a Systemic Inflammatory Disease: Contribution of Liver and Heart to the Pathogenesis.Viruses · 2019Article
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- Immunopathologic Effects of Prednisolone and Cyclosporine A on Feline Immunodeficiency Virus Replication and Persistence.Viruses · 2019Article
- Inflammatory Mediators in the Mesenteric Lymph Nodes, Site of a Possible Intermediate Phase in the Immune Response to Feline Coronavirus and the Pathogenesis of Feline Infectious Peritonitis?Journal of comparative pathology · 2019Article
- Co-infection with feline retrovirus is related to changes in immunological parameters of cats with sporotrichosis.PloS one · 2018Article
- FIV vaccine with receptor epitopes results in neutralizing antibodies but does not confer resistance to challenge.NPJ vaccines · 2018Article
- Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi).Virology journal · 2017Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed real-time PCR systems to quantitate feline cytokine gene expression. The method is based on the cleavage of fluorescent dye-labelled probes by the 5'-3' exonuclease activity of the Taq DNA polymerase during PCR and measurement of fluorescence intensity by a Sequence Detection System. The feline-specific TaqMan probes were designed to encompass an intron, thus allowing differentiation of complementary DNA versus genomic DNA amplification products. Quantitative analysis of cytokine cDNA concentrations was performed in comparison to feline GAPDH. Messenger RNA (mRNA) from the universally expressed housekeeping gene GAPDH proved to be useful as an amplification control and allowed for correction of variations in the efficiencies of RNA extraction and reverse transcription. GAPDH mRNAs were readily detectable in cDNAs prepared from unstimulated feline peripheral blood mononuclear cells (PBMCs) and from frozen cell pellets, while cytokines (Interleukin (IL)-4, IL-10, IL-12 p35, IL-12 p40, IFNgamma, IL-16) were expressed at variable amounts. IFNgamma transcription was found to be upregulated in stimulated PBMCs and feline cell lines. The synthesis of cDNA and the performance of the PCR in separate tubes proved to be of superior sensitivity compared to a single-tube based system. The assays described are highly reproducible, require no post-PCR manipulation of the amplicons and permit the analysis of several hundred PCR reactions per day. With this method it is possible to detect and quantify cytokine mRNA expression reliably in small amounts of cells even after storage of samples for at least 5 years.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.