ArticleBiophysical journal1999
Membrane tether formation from blebbing cells.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
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Who cites it
181 citing papers in PubMed, 433 citations in OpenAlex.
- Piezo2 tension sensitivity and its modulation by alternative splicing.Cell reports · 2026Article
- A biophysical model of phagocytic cup dynamics: The effect of membrane tension.PLoS computational biology · 2026Article
- Piezo2 tension sensitivity and its modulation by alternative splicing.bioRxiv : the preprint server for biology · 2026Article
- Target cell cortical tension regulates macrophage trogocytosis.Nature cell biology · 2025Article
- Adherent cells sustain membrane tension gradients independently of migration.Nature communications · 2025Article
- Membrane Tension Regulation is Required for Wound Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Programmable Lipid Bilayer Tension-Control Apparatus for Quantitative Mechanobiology.ACS nano · 2024Article
- Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow.Current opinion in cell biology · 2024Review
- Research progress on the regulatory role of cell membrane surface tension in cell behavior.Heliyon · 2024Review
- Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow.bioRxiv : the preprint server for biology · 2024Article
- Cell Line and Media Composition Influence the Production of Giant Plasma Membrane Vesicles.ACS biomaterials science & engineering · 2024Article
- Membrane transformations of fusion and budding.Nature communications · 2024Review
- Article
- Three membrane fusion pore families determine the pathway to pore dilation.Biophysical journal · 2023Article
- Sodium Nitroprusside Stimulation of Elastic Matrix Regeneration by Aneurysmal Smooth Muscle Cells.Tissue engineering. Part A · 2023Article
- Extensible membrane nanotubules mediate attachment of Trypanosoma cruzi epimastigotes under flow.PloS one · 2023Article
- Micromechanics of Biomembranes.The Journal of membrane biology · 2022Review
- Force- and cell state-dependent recruitment of Piezo1 drives focal adhesion dynamics and calcium entry.Science advances · 2022Article
- A Theoretical Basis for Nanodomains.The Journal of membrane biology · 2022Review
- Modelling membrane curvature generation using mechanics and machine learning.Journal of the Royal Society, Interface · 2022Article
121 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane tension has been proposed to be important in regulating cell functions such as endocytosis and cell motility. The apparent membrane tension has been calculated from tether forces measured with laser tweezers. Both membrane-cytoskeleton adhesion and membrane tension contribute to the tether force. Separation of the plasma membrane from the cytoskeleton occurs in membrane blebs, which could remove the membrane-cytoskeleton adhesion term. In renal epithelial cells, tether forces are significantly lower on blebs than on membranes that are supported by cytoskeleton. Furthermore, the tether forces are equal on apical and basolateral blebs. In contrast, tether forces from membranes supported by the cytoskeleton are greater in apical than in basolateral regions, which is consistent with the greater apparent cytoskeletal density in the apical region. We suggest that the tether force on blebs primarily contains only the membrane tension term and that the membrane tension may be uniform over the cell surface. Additional support for this hypothesis comes from observations of melanoma cells that spontaneously bleb. In melanoma cells, tether forces on blebs are proportional to the radius of the bleb, and as large blebs form, there are spikes in the tether force in other cell regions. We suggest that an internal osmotic pressure inflates the blebs, and the pressure calculated from the Law of Laplace is similar to independent measurements of intracellular pressures. When the membrane tension term is subtracted from the apparent membrane tension over the cytoskeleton, the membrane-cytoskeleton adhesion term can be estimated. In both cell systems, membrane-cytoskeleton adhesion was the major factor in generating the tether force.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.