ArticleBiophysical journal1999
Hemifusion between cells expressing hemagglutinin of influenza virus and planar membranes can precede the formation of fusion pores that subsequently fully enlarge.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Computational methods to study enveloped viral entry.Biochemical Society transactions · 2021Review
- Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes.International journal of molecular sciences · 2020Article
- Constitutively Expressed IFITM3 Protein in Human Endothelial Cells Poses an Early Infection Block to Human Influenza Viruses.Journal of virology · 2016Article
- Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores.Biophysical journal · 2016Article
- Late stages of the synchronized macrophage fusion in osteoclast formation depend on dynamin.The Biochemical journal · 2014Article
- Molecular dynamics simulation analysis of membrane defects and pore propensity of hemifusion diaphragms.Biophysical journal · 2013Article
- Class II fusion proteins.Advances in experimental medicine and biology · 2013Review
- Rapid membrane fusion of individual virus particles with supported lipid bilayers.Biophysical journal · 2007Article
- Transmembrane peptides stabilize inverted cubic phases in a biphasic length-dependent manner: implications for protein-induced membrane fusion.Biophysical journal · 2006Article
- SNAREs can promote complete fusion and hemifusion as alternative outcomes.The Journal of cell biology · 2005Article
- Imaging individual retroviral fusion events: from hemifusion to pore formation and growth.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The energetics of membrane fusion from binding, through hemifusion, pore formation, and pore enlargement.The Journal of membrane biology · 2004Review
- Common properties of fusion peptides from diverse systems.Bioscience reports · 2000Review
- The transmembrane domain of influenza hemagglutinin exhibits a stringent length requirement to support the hemifusion to fusion transition.The Journal of cell biology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The chronological relation between the establishment of lipid continuity and fusion pore formation has been investigated for fusion of cells expressing hemagglutinin (HA) of influenza virus to planar bilayer membranes. Self-quenching concentrations of lipid dye were placed in the planar membrane to monitor lipid mixing, and time-resolved admittance measurements were used to measure fusion pores. For rhodamine-PE, fusion pores always occurred before a detectable amount of dye moved into an HA-expressing cell. However, with DiI in the planar membrane, the relationship was reversed: the spread of dye preceded formation of small pores. In other words, by using DiI as probe, hemifusion was clearly observed to occur before pore formation. For hemifused cells, a small pore could form and subsequently fully enlarge. In contrast, for cells that express a glycosylphosphatidylinositol-anchored ectodomain of HA, hemifusion occurred, but no fully enlarged pores were observed. Therefore, the transmembrane domain of HA is required for the formation of fully enlarging pores. Thus, with the planar bilayer membranes as target, hemifusion can precede pore formation, and the occurrence of lipid dye spread does not preclude formation of pores that can enlarge fully.
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