ArticleBiophysical journal1999
Elasticity of the red cell membrane and its relation to hemolytic disorders: an optical tweezers study.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 256 citations in OpenAlex.
- Review
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- Optical tweezers in biomedical research - progress and techniques.Journal of medicine and life · 2024Review
- Viscoelastic phenotyping of red blood cells.Biophysical journal · 2024Article
- Tired and stressed: direct holographic quasi-static stretching of aging echinocytes and discocytes in plasma using optical tweezers [Invited].Biomedical optics express · 2024Article
- Measurement of red blood cell deformability during morphological changes using rotating-glass-plate-based scanning optical tweezers.Biomedical optics express · 2023Article
- Melatonin: Regulation of Prion Protein Phase Separation in Cancer Multidrug Resistance.Molecules (Basel, Switzerland) · 2022Review
- Non-Invasive Dynamic Reperfusion of MicrovesselsFrontiers in bioengineering and biotechnology · 2022Article
- The Rise of the OM-LoC: Opto-Microfluidic Enabled Lab-on-Chip.Micromachines · 2021Review
- Deformability of Stored Red Blood Cells.Frontiers in physiology · 2021Review
- Constitutive Model of Erythrocyte Membranes with Distributions of Spectrin Orientations and Lengths.Biophysical journal · 2020Article
- Apparent attenuation by opto-acoustic defocus in phonon microscopy.Photoacoustics · 2020Article
- An Active Biomechanical Model of Cell Adhesion Actuated by Intracellular Tensioning-Taxis.Biophysical journal · 2020Article
- Experimental study of the difference in deformation between normal and pathological, renal and bladder, cells induced by acoustic radiation force.European biophysics journal : EBJ · 2020Article
- Probing force in living cells with optical tweezers: from single-molecule mechanics to cell mechanotransduction.Biophysical reviews · 2019Review
- Enhancement of optical force acting on vesicles via the binding of gold nanoparticles.Royal Society open science · 2019Article
- Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction.Frontiers in bioengineering and biotechnology · 2019Review
- Techniques to stimulate and interrogate cell-cell adhesion mechanics.Extreme Mechanics Letters · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used optical tweezers to study the elasticity of red cell membranes; force was applied to a bead attached to a permeabilized spherical ghost and the force-extension relation was obtained from the response of a second bead bound at a diametrically opposite position. Interruption of the skeletal network by dissociation of spectrin tetramers or extraction of the actin junctions engendered a fourfold reduction in stiffness at low applied force, but only a twofold change at larger extensions. Proteolytic scission of the ankyrin, which links the membrane skeleton to the integral membrane protein, band 3, induced a similar effect. The modified, unlike the native membranes, showed plastic relaxation under a prolonged stretch. Flaccid giant liposomes showed no measurable elasticity. Our observations indicate that the elastic character is at least as much a consequence of the attachment of spectrin as of a continuous membrane-bound network, and they offer a rationale for formation of elliptocytes in genetic conditions associated with membrane-skeletal perturbations. The theory of Parker and Winlove for elastic deformation of axisymmetric shells (accompanying paper) allows us to determine the function BH(2) for the spherical saponin-permeabilized ghost membranes (where B is the bending modulus and H the shear modulus); taking the literature value of 2 x 10(-19) Nm for B, H then emerges as 2 x 10(-6) Nm(-1). This is an order of magnitude higher than the value reported for intact cells from micropipette aspiration. Reasons for the difference are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.