ArticleBiophysical journal1999
Annealing accounts for the length of actin filaments formed by spontaneous polymerization.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Topological control of spontaneous failure in active nematic solids.Nature materials · 2026Article
- 3D printing cytoskeletal networks: ROS-induced filament severing leads to surge in actin polymerization.bioRxiv : the preprint server for biology · 2025Article
- Myosin-based nucleation of actin filaments contributes to stereocilia development critical for hearing.Nature communications · 2025Article
- The mode of subunit addition regulates the processive elongation of actin filaments by formin.The Journal of biological chemistry · 2025Article
- Computational tools for quantifying actin filament numbers, lengths, and bundling.Biology open · 2024Article
- Dynamic interfaces for contact-time control of colloidal interactions.Soft matter · 2023Article
- Molecular Basis for Actin Polymerization Kinetics Modulated by Solution Crowding.Biomolecules · 2023Article
- A statistical mechanics model for determining the length distribution of actin filaments under cellular tensional homeostasis.Scientific reports · 2022Article
- Emergence and maintenance of variable-length actin filaments in a limiting pool of building blocks.Biophysical journal · 2022Article
- Article
- New Insights into the Roles of lncRNAs as Modulators of Cytoskeleton Architecture and Their Implications in Cellular Homeostasis and in Tumorigenesis.Non-coding RNA · 2022Review
- Cooperative bundling by fascin generates actin structures with architectures that depend on filament length.Frontiers in cell and developmental biology · 2022Article
- Mechanism of actin filament nucleation.Biophysical journal · 2021Article
- Nucleation limits the lengths of actin filaments assembled by formin.Biophysical journal · 2021Article
- Directional allosteric regulation of protein filament length.Physical review. E · 2020Article
- The Cytoskeleton of the Retinal Pigment Epithelium: from Normal Aging to Age-Related Macular Degeneration.International journal of molecular sciences · 2019Review
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- The role of annealing and fragmentation in human tau aggregation dynamics.The Journal of biological chemistry · 2019Article
- Triggered disassembly and reassembly of actin networks induces rigidity phase transitions.Soft matter · 2019Article
- Actin Cross-Linking Toxin Is a Universal Inhibitor of Tandem-Organized and Oligomeric G-Actin Binding Proteins.Current biology : CB · 2018Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We measured the lengths of actin filaments formed by spontaneous polymerization of highly purified actin monomers by fluorescence microscopy after labeling with rhodamine-phalloidin. The length distributions are exponential with a mean of approximately 7 microm (2600 subunits). This length is independent of the initial concentration of actin monomer, an observation inconsistent with a simple nucleation-elongation mechanism. However, with the addition of physically reasonable rates of filament annealing and fragmenting, a nucleation-elongation mechanism can reproduce the observed average length of filaments in two types of experiments: 1) filaments formed from a wide range of highly purified actin monomer concentrations, and 2) filaments formed from 24 microM actin over a range of CapZ concentrations.
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