ArticleThe Biochemical journal1999
Expression of glypican-4 in haematopoietic-progenitor and bone-marrow-stromal cells.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- A translational perspective of the malignant hematopoietic proteoglycome.Cell & bioscience · 2025Review
- The extracellular matrix of hematopoietic stem cell niches.Advanced drug delivery reviews · 2022Review
- Wharton's Jelly Mesenchymal Stromal Cells Support the Expansion of Cord Blood-derived CD34Cell transplantation · 2018Article
- Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry.Scientific reports · 2017Article
- Heparan sulfate proteoglycans as key regulators of the mesenchymal niche of hematopoietic stem cells.Glycoconjugate journal · 2017Review
- Unbiased discovery of glypican as a receptor for LRRTM4 in regulating excitatory synapse development.Neuron · 2013Article
- Congenic mice confirm that collagen X is required for proper hematopoietic development.PloS one · 2010Article
- Heparan sulfate proteoglycans: a GAGgle of skeletal-hematopoietic regulators.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Review
- Differential expression of syndecans and glypicans in chronically inflamed synovium.Annals of the rheumatic diseases · 2008Article
- Linking hematopoiesis to endochondral skeletogenesis through analysis of mice transgenic for collagen X.The American journal of pathology · 2002Article
- A dominant interference collagen X mutation disrupts hypertrophic chondrocyte pericellular matrix and glycosaminoglycan and proteoglycan distribution in transgenic mice.The American journal of pathology · 2001Article
- The contribution of in vivo manipulation of gene expression to the understanding of the function of glypicans.Glycoconjugate journalReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heparan sulphate proteoglycans and the extracellular matrix of bone-marrow-stromal cells are important components of the microenvironment of haematopoietic tissues and are involved in the interaction of haematopoietic stem and stromal cells. Previous studies have emphasized the role of heparan sulphate proteoglycan synthesis by bone-marrow-stromal cells. In the present study we describe the expression of glypican-4 (GPC-4), belonging to the glypican family, in bone-marrow-stromal cells and haematopoietic-progenitor cells of human and murine origin. Expression of GPC-4 was shown on the mRNA-level by reverse transcription-PCR and Northern blot analysis. Amplification products were cloned and sequenced, to confirm these results. To analyze the expression of GPC-4 on the protein level, polyclonal antibodies against selected peptides were raised in rabbits. Western blot analysis showed expression of GPC-4 as a heparan sulphate proteoglycan in the human haematopoietic-progenitor cell line TF-1 and normal human bone marrow. These results were confirmed by FACS analysis of TF-1 cells. Furthermore, GPC-4-positive progenitor cells and stromal cells were enriched from normal human bone marrow by magnetic-cell sorting and analysed by confocal laser-scanning microscopy.
Indexed as
Identifiers
10585884PMC1220719W2059057508What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.