ArticleThe Biochemical journal1999
Direct interaction between p47phox and protein kinase C: evidence for targeting of protein kinase C by p47phox in neutrophils.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 81 citations in OpenAlex.
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- Quercetin Ameliorates Neuropathic Pain after Brachial Plexus AvulsionCurrent neuropharmacology · 2023Article
- Toll-like receptor 5 deficiency diminishes doxorubicin-induced acute cardiotoxicity in mice.Theranostics · 2020Article
- Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils.The Journal of experimental medicine · 2019Article
- PKCδ-dependent p47phox activation mediates methamphetamine-induced dopaminergic neurotoxicity.Free radical biology & medicine · 2018Article
- IFN-λ suppresses intestinal inflammation by non-translational regulation of neutrophil function.Nature immunology · 2017Article
- Signalling mechanisms mediating ZnScientific reports · 2017Article
- Role of PKCδ in Enhanced Expression of Gqα/PLCβ1 Proteins and VSMC Hypertrophy in Spontaneously Hypertensive Rats.PloS one · 2016Article
- NOX Activation by Subunit Interaction and Underlying Mechanisms in Disease.Frontiers in cellular neuroscience · 2016Review
- A time course of NADPH-oxidase up-regulation and endothelial nitric oxide synthase activation in the hippocampus following neurotrauma.Free radical biology & medicine · 2014Article
- Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model.Diseases (Basel, Switzerland) · 2013Article
- Bone marrow chimeras in the study of experimental stroke.Translational stroke research · 2012Article
- Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures.Free radical biology & medicine · 2012Article
- Molecular Signals Elicited by GPCR Agonists in Hypertension, Cardiovascular Remodeling: Are MMPs and ADAMs Elusive Therapeutic Targets?Current hypertension reviews · 2012Article
- Biochemistry, physiology, and pathophysiology of NADPH oxidases in the cardiovascular system.Circulation research · 2012Review
- Characterization of superoxide overproduction by the D-Loop(Nox4)-Nox2 cytochrome b(558) in phagocytes-Differential sensitivity to calcium and phosphorylation events.Biochimica et biophysica acta · 2011Article
- Phosphorylation of p22phox on threonine 147 enhances NADPH oxidase activity by promoting p47phox binding.The Journal of biological chemistry · 2010Article
- Regulation of TNF-induced oxygen radical production in human neutrophils: role of delta-PKC.Journal of leukocyte biology · 2010Article
- Apocynin improves outcome in experimental stroke with a narrow dose range.Neuroscience · 2008Article
- Dihydroxyoctadecamonoenoate esters inhibit the neutrophil respiratory burst.Journal of biosciences · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
p47(phox) is an essential component of the NADPH oxidase, and phosphorylation of p47(phox) is associated with activation of the enzyme. Here we have used p47(phox) affinity chromatography to extract a p47(phox) kinase from neutrophil cytosol. The kinase activity was purified by gel filtration and Mini Q chromatography and shown to be indistinguishable from the catalytic fragments of protein kinase C (PKC)-beta(I), -beta(II) and -delta. The C-terminus of p47(phox) represented the site of interaction with PKC. Co-immunoprecipitation experiments revealed that the interaction between PKC isotypes and p47(phox) takes place in intact cells. However PKC-beta and -delta showed different time courses of co-immunoprecipitation, suggesting that the interactions may serve different functions for the various PKC isotypes. Using cells lacking p47(phox), we investigated the functional relevance of the interaction between PKC and p47(phox). Subcellular fractionation revealed an abnormal recruitment of PKC-beta(I) and -beta(II), but not PKC-delta, to particulate fractions in p47(phox)-deficient cells. Phosphorylation of cytosolic proteins was generally increased in stimulated p47(phox)-deficient neutrophils as compared with normal neutrophils. Furthermore, the cytoskeletal protein coronin was not phosphorylated upon stimulation of p47(phox)-deficient neutrophils. These findings were confirmed in an in vitro-reconstituted system using rat brain cytosol in which addition of p47(phox) affected phosphorylation by PKC/PKM (PKM is the catalytic fragment of PKC). These results indicate that p47(phox) can act as a regulator of PKC in neutrophils.
Indexed as
Identifiers
10585874PMC1220709W1975093575What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.