ArticleThe Biochemical journal1999
Insulin stimulates pancreatic-duodenal homoeobox factor-1 (PDX1) DNA-binding activity and insulin promoter activity in pancreatic beta cells.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 83 citations in OpenAlex.
- Insulin: The Friend and the Foe in the Development of Type 2 Diabetes Mellitus.International journal of molecular sciences · 2020Review
- E4orf1 protein reduces the need for endogenous insulin.Nutrition & diabetes · 2019Article
- Notch Signaling in Pancreatic Development.International journal of molecular sciences · 2015Review
- New insights into the architecture of the islet of Langerhans: a focused cross-species assessment.Diabetologia · 2015Review
- Hexane Extract of Orthosiphon stamineus Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells.Diabetes & metabolism journal · 2015Article
- Article
- Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3β in pancreatic β-cells.The Journal of biological chemistry · 2013Article
- Novel insulin sensitizer modulates nutrient sensing pathways and maintains β-cell phenotype in human islets.PloS one · 2013Article
- Glucagon regulates its own synthesis by autocrine signaling.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Paracrine interactions within islets of Langerhans.Journal of molecular neuroscience : MN · 2012Review
- Pdx1 is post-translationally modified in vivo and serine 61 is the principal site of phosphorylation.PloS one · 2012Article
- Methylglyoxal impairs insulin signalling and insulin action on glucose-induced insulin secretion in the pancreatic beta cell line INS-1E.Diabetologia · 2011Article
- Rictor/mTORC2 is essential for maintaining a balance between beta-cell proliferation and cell size.Diabetes · 2011Article
- Glucose regulates steady-state levels of PDX1 via the reciprocal actions of GSK3 and AKT kinases.The Journal of biological chemistry · 2010Article
- Pleiotropic Roles of PDX-1 in the Pancreas.The review of diabetic studies : RDS · 2007Article
- Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of Langerhans.The Journal of biological chemistry · 2005Article
- Increased islet apoptosis in Pdx1+/- mice.The Journal of clinical investigation · 2003Article
- Nicotinic acid-adenine dinucleotide phosphate-sensitive calcium stores initiate insulin signaling in human beta cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis.Molecular genetics and metabolismReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Pancreatic-duodenal homoeobox factor-1 (PDX1) is a homoeodomain transcription factor that plays an important role in linking glucose metabolism in pancreatic beta cells to the regulation of insulin gene transcription. Our previous results indicated that glucose activates PDX1 DNA-binding activity and insulin promoter activity via a stress-activated signalling pathway involving phosphatidylinositol 3-kinase (PtdIns 3-kinase) and stress-activated protein kinase 2 (SAPK2/p38). The present study was undertaken to determine the effects of other metabolizable and non-metabolizable nutrients. The results indicate that non-metabolizable nutrients, with the exception of 2-deoxyglucose, had no effect. Metabolizable nutrients that could stimulate calcium uptake and insulin release were shown to activate both PDX1 and the insulin promoter. The possible role of insulin acting via an autoregulatory loop was therefore examined. Insulin was shown to potently activate PDX1 DNA-binding activity and insulin promoter activity. The effects of insulin were inhibited by the PtdIns 3-kinase inhibitors wortmannin and LY294002 and by the SAPK2 inhibitor SB203580, suggesting that its effects were mediated via activation of PtdIns 3-kinase and SAPK2. Further support for the insulin-mediated activation of SAPK2 came from the observation that both glucose and insulin stimulated the phosphorylation of SAPK2. These results suggest that both glucose and insulin stimulate PDX1 DNA-binding activity and insulin promoter activity via a pathway involving PtdIns 3-kinase and SAPK2.
Indexed as
Identifiers
10585868PMC1220703W2002014786What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.