ArticleGenetics1999
DNA sequence similarity requirements for interspecific recombination in Bacillus.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- Article
- Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC.Nucleic acids research · 2025Article
- Genetic exchange shapes ultra-small Patescibacteria metabolic capacities in the terrestrial subsurface.mSystems · 2025Article
- Unraveling the tempo and mode of horizontal gene transfer in bacteria.Trends in microbiology · 2025Review
- Genomic divergence across the tree of life.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genetic barriers more than environmental associations explain Serratia marcescens population structure.Communications biology · 2024Article
- Dynamics of bacterial recombination in the human gut microbiome.PLoS biology · 2024Article
- Gene flow and species boundaries of the genusmSystems · 2023Article
- Escherichia Coli: What Is and Which Are?Molecular biology and evolution · 2023Article
- Gene flow and introgression are pervasive forces shaping the evolution of bacterial species.Genome biology · 2022Article
- Protoplast fusion in Bacillus species produces frequent, unbiased, genome-wide homologous recombination.Nucleic acids research · 2022Article
- The Landscape of Recombination Events That Create Nonribosomal Peptide Diversity.Molecular biology and evolution · 2021Article
- Surfactin Facilitates Horizontal Gene Transfer inFrontiers in microbiology · 2021Article
- Experimental Evolution ofGenetics · 2020Article
- Consistent Metagenome-Derived Metrics Verify and Delineate Bacterial Species Boundaries.mSystems · 2020Article
- Gene gain and loss push prokaryotes beyond the homologous recombination barrier and accelerate genome sequence divergence.Nature communications · 2019Article
- Article
- Genomic Inference of Recombination-Mediated Evolution in Xanthomonas euvesicatoria and X. perforans.Applied and environmental microbiology · 2018Article
- Modeling Multispecies Gene Flow Dynamics Reveals the Unique Roles of Different Horizontal Gene Transfer Mechanisms.Frontiers in microbiology · 2018Article
- Et tu, Brute? Not Even Intracellular Mutualistic Symbionts Escape Horizontal Gene Transfer.Genes · 2017Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene transfer in bacteria is notoriously promiscuous. Genetic material is known to be transferred between groups as distantly related as the Gram positives and Gram negatives. However, the frequency of homologous recombination decreases sharply with the level of relatedness between the donor and recipient. Several studies show that this sexual isolation is an exponential function of DNA sequence divergence between recombining substrates. The two major factors implicated in producing the recombinational barrier are the mismatch repair system and the requirement for a short region of sequence identity to initiate strand exchange. Here we demonstrate that sexual isolation in Bacillus transformation results almost exclusively from the need for regions of identity at both the 5' and 3' ends of the donor DNA strand. We show that, by providing the essential identity, we can effectively eliminate sexual isolation between highly divergent sequences. We also present evidence that the potential of a donor sequence to act as a recombinogenic, invasive end is determined by the stability (melting point) of the donor-recipient complex. These results explain the exponential relationship between sexual isolation and sequence divergence observed in bacteria. They also suggest a model for rapid spread of novel adaptations, such as antibiotic resistance genes, among related species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.