ArticleGenetics1999
Intron homing with limited exon homology. Illegitimate double-strand-break repair in intron acquisition by phage t4.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Intein Clustering Suggests Functional Importance in Different Domains of Life.Molecular biology and evolution · 2016Article
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Learning to live together: mutualism between self-splicing introns and their hosts.BMC biology · 2011Review
- Redox-responsive zinc finger fidelity switch in homing endonuclease and intron promiscuity in oxidative stress.Current biology : CB · 2011Article
- Mobile DNA elements in T4 and related phages.Virology journal · 2010Review
- Phage T4 mobE promotes trans homing of the defunct homing endonuclease I-TevIII.Nucleic acids research · 2009Article
- An RNA hairpin sequesters the ribosome binding site of the homing endonuclease mobE gene.Journal of bacteriology · 2009Article
- Multiple controls regulate the expression of mobE, an HNH homing endonuclease gene embedded within a ribonucleotide reductase gene of phage Aeh1.Journal of bacteriology · 2007Article
- Insertion of a homing endonuclease creates a genes-in-pieces ribonucleotide reductase that retains function.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Intronless homing: site-specific endonuclease SegF of bacteriophage T4 mediates localized marker exclusion analogous to homing endonucleases of group I introns.Genes & development · 2002Article
- Barriers to intron promiscuity in bacteria.Journal of bacteriology · 2000Review
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The td intron of bacteriophage T4 encodes a DNA endonuclease that initiates intron homing to cognate intronless alleles by a double-strand-break (DSB) repair process. A genetic assay was developed to analyze the relationship between exon homology and homing efficiency. Because models predict exonucleolytic processing of the cleaved recipient leading to homologous strand invasion of the donor allele, the assay was performed in wild-type and exonuclease-deficient (rnh or dexA) phage. Efficient homing was supported by exon lengths of 50 bp or greater, whereas more limited exon lengths led to a precipitous decline in homing levels. However, extensive homology in one exon still supported elevated homing levels when the other exon was completely absent. Analysis of these "one-sided" events revealed recombination junctions at ectopic sites of microhomology and implicated nucleolytic degradation in illegitimate DSB repair in T4. Interestingly, homing efficiency with extremely limiting exon homology was greatly elevated in phage deficient in the 3'-5' exonuclease, DexA, suggesting that the length of 3' tails is a major determinant of the efficiency of DSB repair. Together, these results suggest that illegitimate DSB repair may provide a means by which introns can invade ectopic sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.