ArticleGenetics1999
Role of exonucleolytic degradation in group I intron homing in phage T4.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- The bacteriophage T4 homologous recombination system: mechanism, applications, conservation, and environmental significance.EcoSal Plus · 2025Review
- A temperature-driven DNA discrimination strategy to distinguish E. coli DNA and phage 5hmC-modified DNA.Nucleic acids research · 2025Article
- Systemic Expression, Purification, and Initial Structural Characterization of Bacteriophage T4 Proteins Without Known Structure Homologs.Frontiers in microbiology · 2021Article
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Learning to live together: mutualism between self-splicing introns and their hosts.BMC biology · 2011Review
- Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives.Virology journal · 2010Review
- Mobile DNA elements in T4 and related phages.Virology journal · 2010Review
- Phage T4 mobE promotes trans homing of the defunct homing endonuclease I-TevIII.Nucleic acids research · 2009Article
- Scientific serendipity initiates an intron odyssey.The Journal of biological chemistry · 2009Article
- An RNA hairpin sequesters the ribosome binding site of the homing endonuclease mobE gene.Journal of bacteriology · 2009Article
- Double-strand break repair in bacteriophage T4: recombination effects of 3'-5' exonuclease mutations.Genetics · 2006Article
- Degenerated recognition property of a mitochondrial homing enzyme in the unicellular green alga Chlamydomonas smithii.Plant molecular biology · 2006Article
- Investigation of the mechanism of meiotic DNA cleavage by VMA1-derived endonuclease uncovers a meiotic alteration in chromatin structure around the target site.Eukaryotic cell · 2006Article
- Group I intron homing in Bacillus phages SPO1 and SP82: a gene conversion event initiated by a nicking homing endonuclease.Journal of bacteriology · 2004Article
- Bacteriophage T4 genome.Microbiology and molecular biology reviews : MMBR · 2003Review
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- Mobile self-splicing group I introns from the psbA gene of Chlamydomonas reinhardtii: highly efficient homing of an exogenous intron containing its own promoter.Molecular and cellular biology · 2001Article
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- Barriers to intron promiscuity in bacteria.Journal of bacteriology · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Homing of the phage T4 td intron is initiated by the intron-encoded endonuclease I-TevI, which cleaves the intronless allele 23 and 25 nucleotides upstream of the intron insertion site (IS). The distance between the I-TevI cleavage site (CS) and IS implicates endo- and/or exonuclease activities to resect the DNA segment between the IS and CS. Furthermore, 3' tails must presumably be generated for strand invasion by 5'-3' exonuclease activity. Three experimental approaches were used to probe for phage nucleases involved in homing: a comparative analysis of in vivo homing levels of nuclease-deficient phage, an in vitro assay of nuclease activity and specificity, and a coconversion analysis of flanking exon markers. It was thereby demonstrated that T4 RNase H, a 5'-3' exonuclease, T4 DNA exonuclease A (DexA) and the exonuclease activity of T4 DNA polymerase (43Exo), 3'-5' exonucleases, play a role in intron homing. The absence of these functions impacts not only homing efficiency but also the extent of degradation and flanking marker coconversion. These results underscore the critical importance of the 3' tail in intron homing, and they provide the first direct evidence of a role for 3' single-stranded DNA ends as intermediates in T4 recombination. Also, the involvement of RNase H, DexA, and 43Exo in homing provides a clear example of the harnessing of functions variously involved in phage nucleic acid metabolism for intron propagation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.