ArticleRNA (New York, N.Y.)1999
A limited number of pseudouridine residues in the human atac spliceosomal UsnRNAs as compared to human major spliceosomal UsnRNAs.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- 2'-O-methylation-dependent installation of NNature communications · 2026Article
- Defining expansions and perturbations to the RNA polymerase III transcriptome and epitranscriptome by modified direct RNA nanopore sequencing.Nature communications · 2026Article
- Defining expansions and perturbations to the RNA polymerase III transcriptome and epitranscriptome by modified direct RNA nanopore sequencing.bioRxiv : the preprint server for biology · 2025Article
- Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome.Nature methods · 2024Article
- A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing.Molecular cancer · 2022Article
- Spliceosomal snRNA Epitranscriptomics.Frontiers in genetics · 2021Review
- Identification of U11snRNA as an endogenous agonist of TLR7-mediated immune pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The chemical diversity of RNA modifications.The Biochemical journal · 2019Review
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- The Role of Noncoding RNA Pseudouridylation in Nuclear Gene Expression Events.Frontiers in bioengineering and biotechnology · 2018Review
- Review
- Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA.Nucleic acids research · 2015Article
- Insight into the mechanisms and functions of spliceosomal snRNA pseudouridylation.World journal of biological chemistry · 2014Review
- Post-transcriptional modification of spliceosomal RNAs is normal in SMN-deficient cells.RNA (New York, N.Y.) · 2012Article
- RNA modifications: a mechanism that modulates gene expression.Methods in molecular biology (Clifton, N.J.) · 2010Review
- Minor spliceosome components are predominantly localized in the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines.Biochemistry · 2008Article
- A novel EB-1/AIDA-1 isoform, AIDA-1c, interacts with the Cajal body protein coilin.BMC cell biology · 2005Article
- Functions and mechanisms of spliceosomal small nuclear RNA pseudouridylation.Wiley interdisciplinary reviews. RNAReview
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two forms of spliceosomes were found in higher eukaryotes. The major form contains the U1, U2, U4, U5, and U6 snRNAs; the minor form contains the U11, U12, U4atac, U5, and U6atac snRNAs. Assembly and function of the major form are based on a complex dynamic of UsnRNA-UsnRNA and UsnRNA-pre-mRNA interactions, and the involved UsnRNA segments are highly posttranscriptionally modified in plants and vertebrates. To further characterize the minor form of spliceosomes, we looked for the psi residues in HeLa cells' U11, U12, U4atac, and U6atac snRNAs, using chemical approaches. Four psi residues were detected in total for these four atac UsnRNAs, compared to 20 in their counterparts of the major spliceosomes. The two psi residues detected in U12 are also found in U2 snRNA. One of them belongs to the branch-site-recognition sequence. It forms one of the base pairs that bulge out the A residue, responsible for the nucleophilic attack. Conservation of this strategic psi residue probably reflects a functional role. Another psi residue was detected in a U4atac snRNA segment involved in formation of helix II with U6atac. The fourth one was detected in the additional stem-loop structure present at the 3' end of U6atac snRNA. Differences in psi content of the atac and major UsnRNAs of human cells may participate in the differentiation of the two splicing systems. Based on secondary structure similarity, U2 and U12 snRNAs on the one hand and U4 and U4atac snRNAs on the other hand may share common psi synthases.
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