ArticleRNA (New York, N.Y.)1999
Metastable structures and refolding kinetics in hok mRNA of plasmid R1.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Finding the direct optimal RNA barrier energy and improving pathways with an arbitrary energy model.Bioinformatics (Oxford, England) · 2020Article
- A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNA.eLife · 2019Article
- Self-powered RNA nanomachine driven by metastable structure.Nucleic acids research · 2019Article
- Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency.Molecules (Basel, Switzerland) · 2019Article
- Type I Toxin-Antitoxin Systems: Regulating Toxin Expression via Shine-Dalgarno Sequence Sequestration and Small RNA Binding.Microbiology spectrum · 2018Review
- Mechanistic insights into type I toxin antitoxin systems in Helicobacter pylori: the importance of mRNA folding in controlling toxin expression.Nucleic acids research · 2017Article
- Predicting secondary structural folding kinetics for nucleic acids.Biophysical journal · 2010Article
- Encoding folding paths of RNA switches.Nucleic acids research · 2007Article
- Kinefold web server for RNA/DNA folding path and structure prediction including pseudoknots and knots.Nucleic acids research · 2005Article
- Evolutionary patterns of non-coding RNAs.Theory in biosciences = Theorie in den Biowissenschaften · 2005Article
- Effect of transcription on folding of the Tetrahymena ribozyme.RNA (New York, N.Y.) · 2003Article
- A pH-jump approach for investigating secondary structure refolding kinetics in RNA.Nucleic acids research · 2002Article
- Translational control by delayed RNA folding: identification of the kinetic trap.RNA (New York, N.Y.) · 2001Article
- Design of multistable RNA molecules.RNA (New York, N.Y.) · 2001Article
- The non-coding RNAs as riboregulators.Nucleic acids research · 2001Article
- Coupled nucleotide covariations reveal dynamic RNA interaction patterns.RNA (New York, N.Y.) · 2000Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Programmed cell death by hok/sok of plasmid R1 and pnd/pndB of R483 mediates plasmid maintenance by killing of plasmid-free cells. It has been previously suggested that premature translation of the plasmid-mediated toxin is prevented during transcription of the hok and pnd mRNAs by the formation of metastable hairpins in the mRNA at the 5' end. Here, experimental evidence is presented for the existence of metastable structures in the 5' leader of the hok and pnd mRNAs in vitro. The kinetics of refolding from the metastable to the stable structure in the isolated fragments of the 5' ends of both the hok and pnd mRNAs could be estimated, in agreement with the structural rearrangement in this region, as predicted to occur during transcription and mRNA activation. The refolding rates of hok and pnd structures are slow enough to allow for the formation of downstream hairpin structures during elongation of the mRNAs, which thereby helps to stabilize the metastable structures. Thus, the kinetic refolding parameters of the hok and pnd mRNAs are consistent with the proposal that the metastable structures prevent premature translation and/or antisense RNA binding during transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.