ArticleThe Journal of cell biology1999
A structural model for phosphorylation control of Dictyostelium myosin II thick filament assembly.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Enzyme-Free Phosphorylation with Kinetic Gating in a De Novo Coiled-Coil System.Journal of the American Chemical Society · 2026Article
- PTEN and the PTEN-like phosphatase CnrN have both distinct and overlapping roles in a Dictyostelium chemorepulsion pathway.Journal of cell science · 2024Article
- SuggestingCells · 2024Article
- One must reconstitute the functions of interest from purified proteins.Frontiers in physiology · 2024Review
- Particle-based model of mechanosensory contractility kit assembly.Biophysical journal · 2022Article
- An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.Molecular biology of the cell · 2019Article
- Various Themes of Myosin Regulation.Journal of molecular biology · 2016Review
- Kinetic Monte Carlo simulations of the assembly of filamentous biomacromolecules by dimer addition mechanism.RSC advances · 2015Article
- Association of polyalanine and polyglutamine coiled coils mediates expansion disease-related protein aggregation and dysfunction.Human molecular genetics · 2014Article
- Temperature dependence of myosin-II tail fragment assembly.Journal of muscle research and cell motility · 2008Article
- Protein kinase Cgamma regulates myosin IIB phosphorylation, cellular localization, and filament assembly.Molecular biology of the cell · 2006Article
- Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod.PLoS biology · 2004Article
- Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast.The Journal of cell biology · 2004Article
- Signaling pathways regulating Dictyostelium myosin II.Journal of muscle research and cell motility · 2002Review
- Regulation of nonmuscle myosins by heavy chain phosphorylation.Journal of muscle research and cell motility · 2001Review
- Flightin is essential for thick filament assembly and sarcomere stability in Drosophila flight muscles.The Journal of cell biology · 2000Article
- A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Myosin II thick filament assembly in Dictyostelium is regulated by phosphorylation at three threonines in the tail region of the molecule. Converting these three threonines to aspartates (3 x Asp myosin II), which mimics the phosphorylated state, inhibits filament assembly in vitro, and 3 x Asp myosin II fails to rescue myosin II-null phenotypes. Here we report a suppressor screen of Dictyostelium myosin II-null cells containing 3 x Asp myosin II, which reveals a 21-kD region in the tail that is critical for the phosphorylation control. These data, combined with new structural evidence from electron microscopy and sequence analyses, provide evidence that thick filament assembly control involves the folding of myosin II into a bent monomer, which is unable to incorporate into thick filaments. The data are consistent with a structural model for the bent monomer in which two specific regions of the tail interact to form an antiparallel tetrameric coiled-coil structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.