ArticlePlant molecular biology1999
Nectarin I is a novel, soluble germin-like protein expressed in the nectar of Nicotiana sp.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 96 citations in OpenAlex.
- Influence of drought stress on the metabolite and ion composition in nectar and nectaries of different day- and night-flowering Nicotiana species.Plant biology (Stuttgart, Germany) · 2026Article
- MYC2 and MYC3 orchestrate pith lignification to defend Nicotiana attenuata stems against a stem-boring weevil.The New phytologist · 2025Article
- Nectar biosynthesis is conserved among floral and extrafloral nectaries.Plant physiology · 2021Article
- Metabolomic Profiling ofMetabolites · 2020Article
- Article
- An integrated transcriptomics and metabolomics analysis of thePlant direct · 2019Article
- The major nectar protein of Brassica rapa is a non-specific lipid transfer protein, BrLTP2.1, with strong antifungal activity.Journal of experimental botany · 2018Article
- Nectar Analysis Throughout the GenusFrontiers in plant science · 2018Article
- Proteogenomic Analysis Greatly Expands the Identification of Proteins Related to Reproduction in the Apogamous FernFrontiers in plant science · 2017Article
- Floral Nectary Morphology and Proteomic Analysis of Nectar of Liriodendron tulipifera Linn.Frontiers in plant science · 2016Article
- Versatility of germin-like proteins in their sequences, expressions, and functions.Functional & integrative genomics · 2015Article
- Bean metal-responsive element-binding transcription factor confers cadmium resistance in tobacco.Plant physiology · 2015Article
- Role of proline and GABA in sexual reproduction of angiosperms.Frontiers in plant science · 2015Review
- The F-box protein COI1 functions upstream of MYB305 to regulate primary carbohydrate metabolism in tobacco (Nicotiana tabacum L. cv. TN90).Journal of experimental botany · 2014Article
- Natural variation in floral nectar proteins of two Nicotiana attenuata accessions.BMC plant biology · 2013Article
- Article
- Acidic α-galactosidase is the most abundant nectarin in floral nectar of common tobacco (Nicotiana tabacum).Annals of botany · 2012Article
- Regulation of two germin-like protein genes during plum fruit development.Journal of experimental botany · 2010Article
- Petunia nectar proteins have ribonuclease activity.Journal of experimental botany · 2010Article
- Silencing NaTPI expression increases nectar germin, nectarins, and hydrogen peroxide levels and inhibits nectar removal from plants in nature.Plant physiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified a limited number of proteins secreted into the nectar of tobacco plants. Nectarin I is the most highly expressed nectar protein and has a monomer molecular mass of 29 kDa. The other major nectar proteins are expressed at lower levels and have monomer molecular masses of 41, 54, and 65 kDa respectively. Nectarin I was purified and antiserum was raised against the protein. Under nondenaturing conditions, Nectarin I has an apparent molecular mass of > 120 kDa. The expression of Nectarin I was restricted to nectary tissues and to a much lower level in the ovary. No Nectarin I was found in petals, stems, leaves, or roots or other floral tissues. The expression of Nectarin I was also developmentally regulated. It is expressed in nectary tissues only while nectar is being actively secreted. Subsequently, the N-terminus of purified Nectarin I was sequenced. Sequence identity showed Nectarin I is related to wheat germin. Although hydrogen peroxide is readily detectable in tobacco floral nectar, we were unable to demonstrate any oxalate oxidase activity for Nectarin I. A partial cDNA encoding the mature Nectarin I N-terminus was isolated and used to probe a Nicotiana plumbaginifolia genomic library. The Nectarin I gene was isolated and the translated sequence was consistent with both N-terminal and internal cyanogen bromide-derived amino acid sequence. The gene contains a single 386 nt intron and encodes a mature protein of 197 amino acids.
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