ArticleRNA (New York, N.Y.)1999
Posttranscriptional gene silencing of gn1 in tobacco triggers accumulation of truncated gn1-derived RNA species.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Sense transgene-induced post-transcriptional gene silencing in tobacco compromises the splicing of endogenous counterpart genes.PloS one · 2014Article
- Transgene constructs lacking transcription termination signal induce efficient silencing of endogenous targets in Arabidopsis.Molecular genetics and genomics : MGG · 2009Article
- The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing.The Plant cell · 2003Article
- Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerases.BMC structural biology · 2003Article
- Replicating satellite RNA induces sequence-specific DNA methylation and truncated transcripts in plants.RNA (New York, N.Y.) · 2001Article
- Detailed characterization of the posttranscriptional gene-silencing-related small RNA in a GUS gene-silenced tobacco.RNA (New York, N.Y.) · 2000Article
- RNA degradation and models for post-transcriptional gene-silencing.Plant molecular biology · 2000Review
Corrections and comments
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Authors and funding
5 authors.
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Abstract
Posttranscriptional silencing of basic beta-1,3-glucanase genes in the tobacco line T17 is manifested by reduced transcript levels of the gn1 transgene and homologous, endogenous basic beta-1,3-glucanase genes. An RNA ligation-mediated rapid amplification of cDNA ends (RLM-RACE) technique was used to compare the 3' termini of gn1 RNAs present in expressing (hemizygous and young homozygous) and silenced (mature homozygous) T17 plants. Full-length, polyadenylated gn1 transcripts primarily accumulated in expressing plants, whereas in silenced T17 plants, mainly 3'-truncated, nonpolyadenylated gn1 RNAs were detected. The relative abundance of these 3'-truncated gn1 RNA species gradually increased during the establishment of silencing in homozygous T17 plants. Similar 3'-truncated, nonpolyadenylated gn1 RNA products were observed in an independent case of beta-1,3-glucanase posttranscriptional gene silencing. This suggests that these 3'-truncated gn1 RNAs are a general feature of tobacco plants showing posttranscriptional silencing of the gn1 transgene.
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