ArticleJournal of bacteriology1999
Genetic dissection of the outer membrane secretin PulD: are there distinct domains for multimerization and secretion specificity?
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Type three secretion system in Salmonella Typhimurium: the key to infection.Genes & genomics · 2020Review
- Unraveling the Self-Assembly of themBio · 2017Article
- Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.The Journal of biological chemistry · 2017Article
- Topology and Structure/Function Correlation of Ring- and Gate-forming Domains in the Dynamic Secretin Complex of Thermus thermophilus.The Journal of biological chemistry · 2016Article
- Lipids assist the membrane insertion of a BAM-independent outer membrane protein.Scientific reports · 2015Article
- Article
- Secretion of bacterial lipoproteins: through the cytoplasmic membrane, the periplasm and beyond.Biochimica et biophysica acta · 2014Review
- Sequential steps in the assembly of the multimeric outer membrane secretin PulD.The Journal of biological chemistry · 2013Article
- Assembly of the type II secretion system such as found in Vibrio cholerae depends on the novel Pilotin AspS.PLoS pathogens · 2013Article
- Structure and assembly of a trans-periplasmic channel for type IV pili in Neisseria meningitidis.PLoS pathogens · 2012Article
- The type II secretion system: biogenesis, molecular architecture and mechanism.Nature reviews. Microbiology · 2012Review
- Outer membrane targeting of secretin PulD protein relies on disordered domain recognition by a dedicated chaperone.The Journal of biological chemistry · 2011Article
- Structural and functional studies on the interaction of GspC and GspD in the type II secretion system.PLoS pathogens · 2011Article
- Structure and function of PilQ, a secretin of the DNA transporter from the thermophilic bacterium Thermus thermophilus HB27.The Journal of biological chemistry · 2011Article
- Structure-function relationships of the outer membrane translocon Wza investigated by cryo-electron microscopy and mutagenesis.Journal of structural biology · 2009Article
- Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody.Structure (London, England : 1993) · 2009Article
- Type II secretion system secretin PulD localizes in clusters in the Escherichia coli outer membrane.Journal of bacteriology · 2009Article
- An inhibitor of gram-negative bacterial virulence protein secretion.Cell host & microbe · 2008Article
- Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Bacterial outer membrane secretin PulD assembles and inserts into the inner membrane in the absence of its pilotin.The EMBO journal · 2006Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Linker and deletion mutagenesis and gene fusions were used to probe the possible domain structure of the dodecameric outer membrane secretin PulD from the pullulanase secretion pathway of Klebsiella oxytoca. Insertions of 24 amino acids close to or within strongly predicted and highly conserved amphipathic beta strands in the C-terminal half of the polypeptide (the beta domain) abolished sodium dodecyl sulfate (SDS)-resistant multimer formation that is characteristic of this protein, whereas insertions elsewhere generally had less dramatic effects on multimer formation. However, the beta domain alone did not form SDS-resistant multimers unless part of the N-terminal region of the protein (the N domain) was produced in trans. All of the insertions except one, close to the C terminus of the protein, abolished function. The N domain alone was highly unstable and did not form SDS-resistant multimers even when the beta domain was present in trans. We conclude that the beta domain is a major determinant of multimer stability and that the N domain contributes to multimer formation. The entire or part of the N domain of PulD could be replaced by the corresponding region of the OutD secretin from the pectate lyase secretion pathway of Erwinia chrysanthemi without abolishing pullulanase secretion. This suggests that the N domain of PulD is not involved in substrate recognition, contrary to the role proposed for the N domain of OutD, which binds specifically to pectate lyase secreted by E. chrysanthemi (V. E. Shevchik, J. Robert-Badouy, and G. Condemine, EMBO J. 16:3007-3016, 1997).
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