ArticleProceedings of the National Academy of Sciences of the United States of America1999
A transgenic mouse model to analyze CD8(+) effector T cell differentiation in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 104 citations in OpenAlex.
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- Intestinal CD4Nature communications · 2025Article
- The role of plasmacytoid dendritic cells (pDCs) in immunity during viral infections and beyond.Cellular & molecular immunology · 2024Review
- Appearance of claudin-5Journal of neuroinflammation · 2021Article
- Dynamic chromatin regulatory landscape of human CAR T cell exhaustion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen.The Journal of experimental medicine · 2020Article
- Article
- An intravital window to image the colon in real time.Nature communications · 2019Article
- Mouse Model for Human Vitiligo.Current protocols in immunology · 2019Article
- SVCT2 Is Expressed by Cerebellar Precursor Cells, Which Differentiate into Neurons in Response to Ascorbic Acid.Molecular neurobiology · 2018Article
- NeuroinvasiveFrontiers in immunology · 2018Article
- Heritable Gene Regulation in the CD4:CD8 T Cell Lineage Choice.Frontiers in immunology · 2017Review
- Current status and recent advances of next generation sequencing techniques in immunological repertoire.Genes and immunity · 2016Review
- Real-time tracking of cell cycle progression during CD8+ effector and memory T-cell differentiation.Nature communications · 2015Article
- A silencer-proximal intronic region is required for sustained CD4 expression in postselection thymocytes.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Transcriptional control of CD4 and CD8 coreceptor expression during T cell development.Cellular and molecular life sciences : CMLS · 2013Review
- NKT cell-TCR expression activates conventional T cells in vivo, but is largely dispensable for mature NKT cell biology.PLoS biology · 2013Article
- The epigenetic landscape of lineage choice: lessons from the heritability of CD4 and CD8 expression.Current topics in microbiology and immunology · 2012Review
- Real-time intravital imaging of cancer models.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2011Review
- Maintaining CD4-CD8 lineage integrity in T cells: where plasticity serves versatility.Seminars in immunology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Antigen-specific effector T cells are prerequisite to immune protection, but because of the lack of effector cell-specific markers, their generation and differentiation has been difficult to study. We report that effector cells are highly enriched in a T cell subset that can be specifically identified in transgenic (T-GFP) mice expressing green fluorescent protein (GFP) under control of the murine CD4 promoter and proximal enhancer. Consistent with previous studies of these transcriptional control elements, GFP was strongly and specifically expressed in nearly all resting and short-term activated CD4(+) and CD8(+) T cells. However, when T-GFP mice were challenged with vaccinia virus, allogeneic tumor cells, or staphylococcal enterotoxin A, the cytotoxic and IFN-gamma-producing T cells lost GFP expression. Upon T cell receptor (TCR) ligation by alphaCD3, sorted GFP(+) cells fluxed calcium and proliferated vigorously. In contrast, GFP(-) effector cells showed a diminished calcium flux and did not proliferate. Instead, they underwent apoptosis unless supplied with exogenous IL-2. By reverse transcription-PCR analysis, the GFP(-) cells up-regulated the pro-apoptotic molecule, Fas-L, and down-regulated gene expression of the proximal TCR signaling molecule, CD3zeta, and c-jun, a component of the AP-1 transcription factor. Thus, differential regulation of TCR signaling may explain the divergent responses of naïve and effector T cells to antigen stimulation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.