ArticleProceedings of the National Academy of Sciences of the United States of America1999
Genetic dissection of dome formation in a mammary cell line: identification of two genes with opposing action.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 30 citations in OpenAlex.
- Characterization of the osteoblast-specific transmembrane protein IFITM5 and analysis of IFITM5-deficient mice.Journal of bone and mineral metabolism · 2011Article
- Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The properties of a mammary gland cancer stem cell.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Expression of the mouse fragilis gene products in immune cells and association with receptor signaling complexes.Genes and immunity · 2006Article
- Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Soluble extracts from Helicobacter pylori induce dome formation in polarized intestinal epithelial monolayers in a laminin-dependent manner.Infection and immunity · 2003Article
- Proteomic dissection of dome formation in a mammary cell line.Journal of mammary gland biology and neoplasia · 2002Review
- Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Proteomic dissection of dome formation in a mammary cell line: role of tropomyosin-5b and maspin.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, we extend the study of the genes controlling the formation of domes in the rat mammary cell line LA7 under the influence of DMSO. The role of the rat8 gene has already been demonstrated. We have now studied two additional genes. The first, called 133, is the rat ortholog of the human epithelial membrane protein 3 (EMP3), a member of the peripheral myelin protein 22 (PMP22)/EMP/lens-specific membrane protein 20 (MP20) gene family that encodes for tetratransmembrane proteins; it is expressed in the LA7 line in the absence of DMSO but not in its presence. The second gene is the beta subunit of the amiloride-sensitive Na(+) channel. Studies with antisense oligonucleotides show that the formation of domes is under the control of all three genes: the expression of rat8 is required for both their formation and their persistence; the expression of the Na(+) channel beta subunit is required for their formation; and the expression of gene 133 blocks the expression of the Na(+) channel genes, thus preventing formation of the domes. The formation of these structures is also accompanied by the expression of alpha(6)beta(1) integrin, followed by that of E-cadherin and cytokeratin 8. It appears, therefore, that dome formation requires the activity of the Na(+) channel and the rat8-encoded protein and is under the negative control of gene 133. DMSO induces dome formation by blocking this control.
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