ArticleProceedings of the National Academy of Sciences of the United States of America1999
Human TATA-binding protein-related factor-2 (hTRF2) stably associates with hTFIIA in HeLa cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 117 citations in OpenAlex.
- RNA-seq analysis of wild-type and mutated TBPL1 gene in breast cancer cells lines through CRISPR/Cas9 approach reveals novel molecular signatures.Scientific reports · 2025Article
- Drosophila architectural proteins M1BP and Opbp cooperatively form the active promoter of a ribosomal protein gene.Epigenetics & chromatin · 2025Article
- Functional implications of paralog genes in polyglutamine spinocerebellar ataxias.Human genetics · 2023Review
- TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage.Nature communications · 2020Article
- TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.Stem cell research & therapy · 2020Article
- Basonuclin 1 deficiency causes testicular premature aging: BNC1 cooperates with TAF7L to regulate spermatogenesis.Journal of molecular cell biology · 2020Article
- The RNA Polymerase II Core Promoter inGenetics · 2019Review
- The punctilious RNA polymerase II core promoter.Genes & development · 2017Review
- TRF2 is recruited to the pre-initiation complex as a testis-specific subunit of TFIIA/ALF to promote haploid cell gene expression.Scientific reports · 2016Article
- NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and Proliferation in the Planarian Schmidtea mediterranea.PLoS genetics · 2016Article
- TBP-like protein (TLP) interferes with Taspase1-mediated processing of TFIIA and represses TATA box gene expression.Nucleic acids research · 2015Article
- Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.Transcription · 2015Article
- TRF2: TRansForming the view of general transcription factors.Transcription · 2015Review
- Drosophila TRF2 is a preferential core promoter regulator.Genes & development · 2014Article
- TRF2 and the evolution of the bilateria.Genes & development · 2014Article
- TRF2, but not TBP, mediates the transcription of ribosomal protein genes.Genes & development · 2014Article
- Activity of the upstream TATA-less promoter of the p21(Waf1/Cip1) gene depends on transcription factor IIA (TFIIA) in addition to TFIIA-reactive TBP-like protein.The FEBS journal · 2014Article
- Article
- Taf7l cooperates with Trf2 to regulate spermiogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- TATA-binding protein (TBP)-like protein is required for p53-dependent transcriptional activation of upstream promoter of p21Waf1/Cip1 gene.The Journal of biological chemistry · 2012Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The TATA-binding protein (TBP)-related factor TRF1, has been described in Drosophila and a related protein, TRF2, has been found in a variety of higher eukaryotes. We report that human (h)TRF2 is encoded by two mRNAs with common protein coding but distinct 5' nontranslated regions. One mRNA is expressed ubiquitously (hTRF2-mRNA1), whereas the other (hTRF2-mRNA2) shows a restricted expression pattern and is extremely abundant in testis. In addition, we show that hTRF2 forms a stable stoichiometric complex with hTFIIA, but not with TAFs, in HeLa cells stably transfected with flag-tagged hTRF2. Neither recombinant human (rh)TRF2 nor the native flag.hTRF2-TFIIA complex is able to replace TBP or TFIID in basal or activated transcription from various RNA polymerase II promoters. Instead, rhTRF2, but not the flag.hTRF2-TFIIA complex, moderately inhibits basal or activated transcription in the presence of rhTBP or flag.TFIID. This effect is either completely (TBP-mediated transcription) or partially (TFIID-mediated transcription) counteracted by addition of free TFIIA. Neither rhTRF2 nor flag. hTRF2-TFIIA has any effect on the repression of TFIID-mediated transcription by negative cofactor-2 (NC2) and neither substitutes for TBP in RNA polymerase III-mediated transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.