ArticleInfection and immunity1999
Misexpression of the opaque-phase-specific gene PEP1 (SAP1) in the white phase of Candida albicans confers increased virulence in a mouse model of cutaneous infection.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 121 papers.
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Who cites it
121 citing papers in PubMed.
- Insights on molecular drivers of phenotypic plasticity in Candidozyma auris.World journal of microbiology & biotechnology · 2026Review
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- Genetic and environmental control of parasexual reproduction in the pathogenicMicrobiology and molecular biology reviews : MMBR · 2025Review
- Transcriptional control ofmBio · 2025Article
- The impact of phenotypic heterogeneity on fungal pathogenicity and drug resistance.FEMS microbiology reviews · 2025Review
- A single gene mutation underpins metabolic adaptation and acquisition of filamentous competence in the emerging fungal pathogen Candida auris.PLoS pathogens · 2024Article
- Review
- White-opaque switching inMicrobiology and molecular biology reviews : MMBR · 2024Review
- Characterization of intestinal fungal community diversity in people living with HIV/AIDS (PLWHA).AIDS research and therapy · 2024Article
- The defective gut colonization ofVirulence · 2023Article
- Article
- Morphogenic plasticity: the pathogenic attribute of Candida albicans.Current genetics · 2023Review
- More than Just Protein Degradation: The Regulatory Roles and Moonlighting Functions of Extracellular Proteases Produced by Fungi Pathogenic for Humans.Journal of fungi (Basel, Switzerland) · 2023Review
- Farnesol and phosphorylation of the transcriptional regulator Efg1 affect Candida albicans white-opaque switching rates.PloS one · 2023Article
- Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia.Journal of clinical laboratory analysis · 2022Article
- Bursts of Genomic Instability Potentiate Phenotypic and Genomic Diversification inFrontiers in genetics · 2022Article
- Role of themBio · 2021Article
- The Glyoxylate Cycle Is Involved in White-Opaque Switching inJournal of fungi (Basel, Switzerland) · 2021Article
- Recording of DNA-binding events reveals the importance of a repurposed Candida albicans regulatory network for gut commensalism.Cell host & microbe · 2021Article
61 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
Candida albicans WO-1 switches reversibly and at high frequency between a white and an opaque colony-forming phenotype that includes dramatic changes in cell morphology and physiology. A misexpression strategy has been used to investigate the role of the opaque-phase-specific gene PEP1 (SAP1), which encodes a secreted aspartyl proteinase, in the expression of the unique opaque-phase phenotype and phase-specific virulence in two animal models. The PEP1 (SAP1) open reading frame was inserted downstream of the promoter of the white-phase-specific gene WH11 in the transforming vector pCPW7, and the resulting transformants were demonstrated to misexpress PEP1 (SAP1) in the white phase. Misexpression did not confer any of the unique morphological characteristics of the opaque phase to cells in the white phase and had no effect on the switching process. However, misexpression conferred upon white-phase cells the increased capacity of opaque-phase cells to grow in medium in which protein was the sole nitrogen source. Misexpression of PEP1 (SAP1) had no effect on the virulence of white-phase cells in a systemic mouse model, in which white-phase cells were already more virulent than opaque-phase cells. Misexpression did, however, confer upon white-phase cells the dramatic increase in colonization of skin in a cutaneous mouse model that was exhibited by opaque-phase cells. Misexpression of PEP1 (SAP1) conferred upon white-phase cells two dissociable opaque-phase characteristics: increased adhesion and the capacity to cavitate skin. The addition of pepstatin A to the cutaneous model inhibited the latter, but not the former, suggesting that the latter is effected by released enzyme, while the former is effected by cell-associated enzyme.
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