ArticleInfection and immunity1999
WdChs4p, a homolog of chitin synthase 3 in Saccharomyces cerevisiae, alone cannot support growth of Wangiella (Exophiala) dermatitidis at the temperature of infection.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- Regulation of melanin production in fungi.Frontiers in fungal biology · 2025Review
- Topography of UV-Melanized Thalli ofPlants (Basel, Switzerland) · 2023Article
- Melanin deposition in twoThe Journal of biological chemistry · 2020Article
- The structural unit of melanin in the cell wall of the fungal pathogenThe Journal of biological chemistry · 2019Article
- Cell-wall dyes interfere with Cryptococcus neoformans melanin deposition.Microbiology (Reading, England) · 2018Article
- Functions of fungal melanin beyond virulence.Fungal biology reviews · 2017Article
- A flavoprotein supports cell wall properties in the necrotrophic fungusFungal biology and biotechnology · 2017Article
- Activation of Melanin Synthesis in Alternaria infectoria by Antifungal Drugs.Antimicrobial agents and chemotherapy · 2015Article
- Fungal Melanin: What do We Know About Structure?Frontiers in microbiology · 2015Review
- Chitin synthases with a myosin motor-like domain control the resistance of Aspergillus fumigatus to echinocandins.Antimicrobial agents and chemotherapy · 2012Article
- Synthesis and assembly of fungal melanin.Applied microbiology and biotechnology · 2012Review
- Regulation of expression, activity and localization of fungal chitin synthases.Medical mycology · 2012Review
- Roles of the pH signaling transcription factor PacC in Wangiella (Exophiala) dermatitidis.Fungal genetics and biology : FG & B · 2009Article
- Immunoaffinity purification of the class V chitin synthase of Wangiella (Exophiala) dermatitidis.Preparative biochemistry & biotechnology · 2009Article
- The Magnaporthe grisea class VII chitin synthase is required for normal appressorial development and function.Molecular plant pathology · 2009Article
- Cytolocalization of the class V chitin synthase in the yeast, hyphal and sclerotic morphotypes of Wangiella (Exophiala) dermatitidis.Fungal genetics and biology : FG & B · 2009Article
- New biosynthetic step in the melanin pathway of Wangiella (Exophiala) dermatitidis: evidence for 2-acetyl-1,3,6,8-Tetrahydroxynaphthalene as a novel precursor.Eukaryotic cell · 2008Article
- Molecular cloning and characterization of WdTUP1, a gene that encodes a potential transcriptional repressor important for yeast-hyphal transitions in Wangiella (Exophiala) dermatitidis.Fungal genetics and biology : FG & B · 2008Article
- WdChs1p, a class II chitin synthase, is more responsible than WdChs2p (Class I) for normal yeast reproductive growth in the polymorphic, pathogenic fungus Wangiella (Exophiala) dermatitidis.Archives of microbiology · 2006Article
- Expression of asexual developmental regulator gene abaA is affected in the double mutants of classes I and II chitin synthase genes, chsC and chsA, of Aspergillus nidulans.Current genetics · 2005Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
By using improved transformation methods for Wangiella dermatitidis, and a cloned fragment of its chitin synthase 4 structural gene (WdCHS4) as a marking sequence, the full-length gene was rescued from the genome of this human pathogenic fungus. The encoded chitin synthase product (WdChs4p) showed high homology with Chs3p of Saccharomyces cerevisiae and other class IV chitin synthases, and Northern blotting showed that WdCHS4 was expressed at constitutive levels under all conditions tested. Reduced chitin content, abnormal yeast clumpiness and budding kinetics, and increased melanin secretion resulted from the disruption of WdCHS4 suggesting that WdChs4p influences cell wall structure, cellular reproduction, and melanin deposition, respectively. However, no significant loss of virulence was detected when the wdchs4Delta strain was tested in an acute mouse model. Using a wdchs1Delta wdchs2Delta wdchs3Delta triple mutant of W. dermatitidis, which grew poorly but adequately at 25 degrees C, we assayed WdChs4p activity in the absence of activities contributed by its three other WdChs proteins. Maximal activity required trypsin activation, suggesting a zymogenic nature. The activity also had a pH optimum of 7.5, was most stimulated by Mg(2+), and was more inhibited by polyoxin D than by nikkomycin Z. Although the WdChs4p activity had a broad temperature optimum between 30 to 45 degrees C in vitro, this activity alone did not support the growth of the wdchs1Delta wdchs2Delta wdchs3Delta triple mutant at 37 degrees C, a temperature commensurate with infection.
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