ArticleInfection and immunity1999
Microtubules are associated with intracellular movement and spread of the periodontopathogen Actinobacillus actinomycetemcomitans.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- The intracellular bacterium Orientia tsutsugamushi uses the autotransporter ScaC to activate BICD adaptors for dynein-based motility.Nature communications · 2025Article
- Article
- Orientia tsutsugamushi: A neglected but fascinating obligate intracellular bacterial pathogen.PLoS pathogens · 2017Review
- Aggregatibacter actinomycetemcomitans - a tooth killer?Journal of clinical and diagnostic research : JCDR · 2014Review
- Serogroup-specific interaction of Neisseria meningitidis capsular polysaccharide with host cell microtubules and effects on tubulin polymerization.Infection and immunity · 2014Article
- Azithromycin kills invasive Aggregatibacter actinomycetemcomitans in gingival epithelial cells.Antimicrobial agents and chemotherapy · 2013Article
- EseG, an effector of the type III secretion system of Edwardsiella tarda, triggers microtubule destabilization.Infection and immunity · 2010Article
- Beyond good and evil in the oral cavity: insights into host-microbe relationships derived from transcriptional profiling of gingival cells.Journal of dental research · 2008Review
- Intracellular survival and vascular cell-to-cell transmission of Porphyromonas gingivalis.BMC microbiology · 2008Article
- Exit from host cells by the pathogenic parasite Toxoplasma gondii does not require motility.Eukaryotic cell · 2008Article
- Phagocytosis of the Lyme disease spirochete, Borrelia burgdorferi, by cells from the ticks, Ixodes scapularis and Dermacentor andersoni, infected with an endosymbiont, Rickettsia peacockii.Journal of insect science (Online) · 2007Article
- Porphyromonas gingivalis vesicles enhance attachment, and the leucine-rich repeat BspA protein is required for invasion of epithelial cells by "Tannerella forsythia".Infection and immunity · 2006Article
- Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells.Infection and immunity · 2006Article
- Nocodazole inhibits macronuclear infection with Holospora obtusa in Paramecium caudatum.Protoplasma · 2005Article
- Expression of major histocompatibility complex class II and CD80 by gingival epithelial cells induces activation of CD4+ T cells in response to bacterial challenge.Infection and immunity · 2005Article
- Shigella deliver an effector protein to trigger host microtubule destabilization, which promotes Rac1 activity and efficient bacterial internalization.The EMBO journal · 2002Article
- Oral bacterial disease and the science of cellular conversation.Journal of the Royal Society of Medicine · 2002Review
- Intracellular Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in buccal epithelial cells collected from human subjects.Infection and immunity · 2001Article
- Microtubule- and dynein-mediated movement of Orientia tsutsugamushi to the microtubule organizing center.Infection and immunity · 2001Article
- Burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading.Infection and immunity · 2000Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Actinobacillus actinomycetemcomitans SUNY 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. Internalized organisms also egress from host cells into the assay medium via protrusions that are associated with just a single epithelial cell. Here we demonstrate that agents which inhibit microtubule polymerization (e.g., colchicine) and those which stabilize polymerized microtubules (e.g., taxol) both increase markedly the number of intracellular A. actinomycetemcomitans organisms. Furthermore, both colchicine and taxol prevented the egression of A. actinomycetemcomitans from host cells into the assay medium. Immunofluorescence microscopy revealed that protrusions that mediate the bacterial spread contain microtubules. A. actinomycetemcomitans SUNY 465 and 652, strains that are both invasive and egressive, interacted specifically with the plus ends (growing ends) of the filaments of microtubule asters in a KB cell extract. By contrast, neither A. actinomycetemcomitans 523, a strain that is invasive but not egressive, nor Haemophilus aphrophilus, a noninvasive oral bacterium with characteristics similar to those of A. actinomycetemcomitans, bound to microtubules. Together these data suggest that microtubules function in the spread and movement of A. actinomycetemcomitans and provide the first evidence that host cell dispersion of an invasive bacterium may involve the usurption of host cell microtubules.
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